PsbA, D1 protein of PSII, C-terminal (100 µl)

• Catalog number
  AS01 016
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Liquid in PBS pH 8.0, 0.02% sodium azide
• Immunogen
  KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
• Raised in
  Hen
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Total IgY
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  store at 4°C; make aliquots to avoid working with a stock. Please, Remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB)
• Connected products
  AS01 016S | PsbA protein standard for a quantitative western blotAS05 084 | anti-PsbA C-terminal, rabbit antibody (100 µl)AS11 1786 | anti-PsbA N-terminal, rabbit antibodyAS10 704 | PsbA | D1 protein of PSII, DE-loop, rabbit antibodyAS13 2669 | PsbA | D1 protein of PSII, phosphorylated, rabbit antibodyrecommended secondary antibodyPlant and algla protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1:4000 - 1: 8000 on 5 µg of total protein, detected with standard ECL (WB)
• Molecular weight expected аpparent
  38 | 28-30 kDa
• Verified reactivity
  Alaria esculenta, Amphidinium carterae, Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlamydomonas raudensis (both Antarctic and mesophilic strains), Cyanophora sp. , Gonyaulax polyedra, Fucus vesiculosus, Horderum vulgare, Lobaria pulmonaria, Petunia sp. , Pinus sylvestris, Spartina alterniflora, Synechococcus sp. PCC 7942, Triticum aestivum, Ulva sp., symbiotic dinoflagellates of Stylophora pistillata and Turbinaria reniformis.
• Possible reactivity
  dicots including legumes, monocots, conifers, brown algae, red algae, cryptomonads, stramenopiles, euglenoids, xantophytes, prochlorophytes
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions.In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.This antibody will also detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.
• References
  Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675. Su et al. (2014). Exogenous progesterone alleviates heat and high light stress-induced inactivation of photosystem II in wheat by enhancing antioxidant defense and D1 protein stability. Plant Growth Regul DOI 0.1007/s10725-014-9920-1 Esparza et al. (2013). Katanin Localization Requires Triplet Microtubules in Chlamydomonas reinhardtii. PLOS one. Hoogenboom et al. (2012). Effects of Light, Food Availability and Temperature Stress on the Function of Photosystem II and Photosystem I of Coral Symbionts. POLS one.Morash et al. (2007). Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Canadian J. of Botany, 2007, 85(5): 476-483, 10.1139/B07-043.
• Scientific context
  The PsbA (D1) protein of Photosystem II is rapidly cycled under illumination in all oxygenic photobionts. Disruption of PsbA cycling or losses of PsbA pools are central to photoinhibition of photosynthesis in cyanobacteria, algae and plants under a wide range of conditions including excess light, low temperature and UV exposure. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.
• Notes
  A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and 16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands. Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.Example of a simulataneous western blot detection with RbcL, PsbA and PsaC antibodies.
• Protein number
  P83755 , P0C434 , Q14FH6 , P14660 , P14660
• TAIR number
  ATCG00020
• Hyperlink

• Gene target
  PsbA   protein   PSII   100  
• Short name
  PsbA, D1 protein of PSII, C-terminal (100 µl)
• Alternative name
  PsbA, D1 protein on PSII, C-terminal (100 µl)

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