Lhcbm5, chlorophyll a-b binding protein of LHCII

• Catalog number
  AS09 408
• Price
  Please ask
• Size
  50 µl

• Available ordering format
  Lyophilized
• Immunogen
  SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 50 µl of sterile water
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  Western blot (WB)
• Connected products
  AS06 117 | anti-Lhcb4 | CP29 (Lhcb4) rabbit antibodyAS09 407 | anti-Lhcb5 CP26 rabbit antibodyCollection of antibodies to Chlamydomonas proteinsPlant and algal protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 5000 - 1: 10 000 with ECL (WB)
• Molecular weight expected аpparent
  29 | 30 kDa
• Verified reactivity
  Chlamydomonas reinhardtii
• Possible reactivity
  Chlamydomonas reinhardtii
• No reactivity
  n.a.
• Supplementary information
  for western blot detection image please refer to the article below
• References
  Jeong et al. (2016). Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtii. Biochim Biophys Acta. 2016 Oct 17;1858(1):45-55. doi: 10.1016/j.bbabio.2016.10.007. Grewe et al. (2014). Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii. Plant Cell. 2014 Apr 4;26(4):1598-1611.
• Scientific context
  Lhcbm5 is one of minor LHCII that associates with PSI in state-2
• Notes
  This antibody cross-reacts with three major LHCII proteins of Chlamydomonas, which are slightly smaller than Lhcam5 on SDS-gel. 6M urea SDS-PAGE is one of the best systems that separate Lhcbm5 and the other major LHCII proteins. The dilution of the antibody should be carefully determined to reduce the cross-reactions with other major LHCII proteins, we recommend for this purpose to use the dilution of 1: 10 000- 1: 50 000
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink

• Gene target
  Lhcbm5   chlorophyll   a-b   binding   protein   LHCII  
• Short name
  Lhcbm5, chlorophyll a-b binding protein of LHCII
• Alternative name
  Lhcbm5, chlorophyll a-b binding protein on LHCII

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