Lhcb1, LHCII type I chlorophyll a/b-binding protein (100 µg)

• Catalog number
  AS01 004-100
• Price
  Please ask
• Size
  2 x 50 µg

• Available ordering format
  Lyophilized
• Immunogen
  BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb1 proteins from angiosperms (monocots and dicots) and gymnosperms, includingArabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430 (Lhcb1.4), and At2g34420 (Lhcb1.5)
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified serum
• How to reconstitute
  For reconstitution add 50 µl of sterile water to each tube.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB)
• Connected products
  AS01 004 | Lhcb1 | LHCII type I chlorophyll a/b-binding proteinAS01 004PRE | Lhcb1 | LHCII type I chlorophyll a/b-binding protein, pre-immune serumAS01 011 | 2 | Set of 10 plant anti-Lhca and anti-Lhcb antibodiesAS01 011 CHLAMYDOMONAS | 2 | Set of 4 Chlamydomonas anti-Lhc antibodiesPlant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1 : 2000, detected with standard ECL (WB)
• Molecular weight expected аpparent
  25 | 25 kDa for Arabidopsis thaliana
• Verified reactivity
  Arabidopsis thaliana, Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Silene vulgaris, Spinacia oleracea, Zea mays
• Possible reactivity
  Triticum aestivum, Vitis vinifera, angiosperms (monocots and dicots), gymnosperms, mosses
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Protein is processed into mature form (Jansson 1999).
• References
  Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.
• Scientific context
  The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467-9476
• Notes
  None
• Protein number
  Q8VZ87 , P04777 , P04778 , Q9C5R6 , Q39141
• TAIR number
  AT1G29910
• Hyperlink

• Gene target
  Lhcb1   LHCII   chlorophyll   a/b-binding   protein   100  
• Short name
  Lhcb1, LHCII I chlorophyll a/b-binding protein (100 µg)
• Alternative name
  Lhcb1, LHCII classification I chlorophyll a/b-binding protein (100 µg)

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