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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430 (Lhcb1.4), and At2g34420 (Lhcb1.5)
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 100 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS01 002 Lhcb3 | LHCII type III chlorophyll a/b-binding proteinAS01 003 Lhcb2 | LHCII type II chlorophyll a/b-binding proteinLHC available antibodies against pigment-binding proteinsPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1 : 2500 - 1: 5000 with standard ECL (WB)
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Molecular weight expected аpparent
28 | 25 kDa for Arabidopsis thaliana
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Verified reactivity
Arabidopsis thaliana, Digitaria sanguinalis, Echinochloa crus-galli, Pinus strobus
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Possible reactivity
mainly dicots and some monocots, mosses, green algae
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This Lhcb1 antibody is directed specifically against the Arabidopsis Lhcb1 gene products, for those that would prefer higher specific activity over broader specificity offered by Agrisera older Lhcb1 antibody, AS01 004Protein is processed into mature form (Jansson 1999).
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References
Correa-Galvis et al. (2016). PsbS interactions involved in the activation of energy dissipation in Arabidopsis. Nature Plants 2, Article number: 15225 (2016) doi:10.1038/nplants.2015.225 Longoni et al. (2015). Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions. Plant Physiol. 2015 Oct 5. pii: pp.01498.2015.Wientjes et al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.
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Scientific context
The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.
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Notes
A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467-9476
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Protein number
Q8VZ87 , P04777 , P04778 , Q9C5R6 , Q39141
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TAIR number
AT1G29910, AT1G29920, AT1G29930, AT2G34420, AT2G34430
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