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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
BSA-conjugated synthetic peptide derived from the Lhca2 protein of Arabidopsis thaliana UniProt: Q9SYW8, Q8LCQ4, TAIR: At3g61470. This sequence is highly conserved in Lhca2 proteins of angiosperms (monocots and dicots) and gymnosperms as well as in At1g19150. This gene codes for the very low expressed Lhca6 protein which also has been denoted as Lhca2*1.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgG
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How to reconstitute
For reconstitution add 200 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodiesAS01 011 Chlamydomonas | A set of anti-Lhc antibodies for ChlamydmonasAvailable antibodies against pigment-binding proteins- LHCrecommended secondary antibodyPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1 : 2000 - 1: 5000, detected with standard ECL (WB)
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Molecular weight expected аpparent
27.7 | 24 kDa for Arabidopsis thaliana
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Verified reactivity
Arabidopsis thaliana, Arachis hypogaea, Bryopsis corticulans, Colobanthus quitensis Kunt Bartl, Chlamydomonas reinhardti (one Lhca-type), Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Physcomitrella patens, Pinus banksiana (the higher of the two bands detected at 24 and 30 kDa is not considered to be specific to any Lhc protein), Prasinoderma sp., Pyramimonas sp. Spinacia oleracea, Zea mays
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Possible reactivity
angiosperms (monocots and dicots), gymnosperms
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
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References
Kunugi et al. (2016). Evolution of Green Plants Accompanied Changes in Light-Harvesting Systems. Plant Cell Physiol. 2016 Apr 6. pii: pcw071. Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.
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Scientific context
The light-harvesting protein Lhca2 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca2 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca2 forms a heterodimer (LHCI-680) with Lhca3 that associates with the PSI core close to PsaF and PsaK.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073.
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Notes
Antibody format is a total IgG fraction, which means that it is a pool of polyclonal antibodies obtained by purification of serum on Protein G, not on a specific antigen column.
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Protein number
Q9SYW8 , Q8LCQ4
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TAIR number
AT3G61470
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