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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
BSA-conjugated synthetic peptide derived from the Lhca1 protein of Arabidopsis thaliana UniProt: Q01667, TAIR: At3g54890. This sequence is highly conserved in Lhca1 proteins of angiosperms (monocots and dicots) and gymnosperms.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgG
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How to reconstitute
For reconstitution add 200 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodiesAS01 011 Chlamydomonas | A set of anti-Lhc antibodies for ChlamydmonasAvailable antibodies against pigment-binding proteins- LHCrecommended secondary antibodyPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1 : 2000 - 1 : 5000, detected with standard ECL (WB)
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Molecular weight expected аpparent
25.99 | 22 kDa for Arabidopsis thaliana
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Verified reactivity
Arabidopsis thaliana, Arachis hypogaea, Bryopsis corticulans, Colobanthus quitensis Kunt Bartl, Guzmania hybrid, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicum), Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Pinus strobus, Pisum sativum, Phaseolus vulgaris, Spinacia oleracea, Zea mays,
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Possible reactivity
angiosperms (monocots and dicots), gymnosperms
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Protein is processed into mature form (Jansson 1999).
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References
Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. Mazur et al. BMC Plant Biology (2016) 16:191. Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113 Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12. Saito et al. (2014). Fe deficiency induces phosphorylation and translocation of Lhcb1 in barley thylakoid membranes. FEBS Lett. 2014 May 8. pii: S0014-5793(14)00317-2. doi: 10.1016/j.febslet.2014.04.031.
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Scientific context
The light-harvesting protein Lhca1 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca1 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca1 forms a heterodimer (LHCI-730) with Lhca4 that associates with the PSI core close to PsaG and PsaF.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073
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Notes
Antibody format is a total IgG fraction, which means that it is a pool of polyclonal antibodies obtained by purification of serum on Protein G, not on a specific antigen column.
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Protein number
Q01667
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TAIR number
AT3G54890
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