IAA, indole-3-acetic acid (N1) (for immunolocalization)

• Catalog number
  AS09 421
• Price
  Please ask
• Size
  200 µg

• Available ordering format
  Inquire
• Immunogen
  BSA-conjugated, via N1 group of indole, indole-3-acetic acid (N1)
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  freeze upol arrival and store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  immunolocalization (IL)
• Connected products
  AS06 193| anti- IAAAS09 420 | anti- indole-3-acetic acid (C1') for ELISASecondary antibodies
• Recommended dilutions for use
  specific information about dilution is going to be included on the vial
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  indole-3-acetic acid (N1) in Eucalyptus globulus, Pinus radiata
• Possible reactivity
  indole-3-acetic acid (N1)
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Antibody can be used for direct detection of free IAA (see reference below)Antibody is provided in 50% glycerol. For larger quantity (1 mg) or for ELISA kit - please inquire.
• References
  Escandon et al. (2016). Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata. Tree Physiol. 2016 Jan;36(1):63-77. doi: 10.1093/treephys/tpv127. Epub 2016 Jan 12.Jesus et al. (2015). Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit. Environ and Exp Botany, Volume 118, October 2015, Pages 56-66. De Diego et al. (2013). Immunolocalization of IAA and ABA in roots and needles of radiata pine (Pinus radiata) during drought and rewatering. Tree Physiol., May; 33(5):537-549.
• Scientific context
  Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.
• Notes
  useful references for immunolocalization work: DeWitte et al. (1999). Dynamics of cytokinins in apical shoot meristems of a day neutral tobacco during floral transition and flower formation. Plant Physiol. 119:111-121. Shiozaki et al. (1999). Localisation of abscisic acid in grape berry by immunohitochemical techniques. J.Japan Hort.Sci.68:1-9. Skene et al. (1987). Model systems for the immunolocalisation of cis, trans-abscisic acid in plant tissues. Planta 172:192-199. Sosountozov et al. (1986). Immunoelectron-microscopy localisation of abscisic acid with collodial gold on Lowicryl-embeded tissues of Chenopodium polyspermum L. Planta 168: 471-481. Zavala et al. (1983). Localisation of a phytohormone using immunocytochemistry. J.Cell Biol. 97:1235-1239.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink

• Gene target
  IAA   indole-3-acetic   acid   for   immunolocalization  
• Gene symbol
  MIR1302-3, MIR509-3, MIR7-3, PIRC13, PIRC11, PIRC14, PIRC12
• Short name
  IAA, indole-3-acetic acid (N1) (for immunolocalization)
• Alternative name
  IAA, indole-3-acetic acid (N1) (to measure immunolocalization)

Gene info

• Identity
  HGNC:35295
• Gene
  MIR1302-3
• Long gene name
  microRNA 1302-3
• Synonyms gene
  • MIRN1302-3
• Synonyms
  • hsa-mir-1302-3
• Locus
  2q14.1
• Discovery year
  2008-11-03
• Entrez gene record
  100302128
• RefSeq identity
  • NR_031632
• Classification
  • MicroRNA MIR1302 family

Gene info

• Identity
  HGNC:33675
• Gene
  MIR509-3
• Long gene name
  microRNA 509-3
• Synonyms gene
  • MIRN509-3
• Synonyms
  • hsa-mir-509-3
• Locus
  Xq27.3
• Discovery year
  2007-10-24
• Entrez gene record
  100126337
• RefSeq identity
  • NR_030629
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:31640
• Gene
  MIR7-3
• Long gene name
  microRNA 7-3
• Synonyms gene
  • MIRN7-3
• Synonyms
  • hsa-mir-7-3
• Locus
  19p13.3
• Discovery year
  2004-04-23
• Entrez gene record
  407045
• RefSeq identity
  • NR_029607
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:37517
• Gene
  PIRC13
• Long gene name
  piwi-interacting RNA cluster 13
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313925
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37515
• Gene
  PIRC11
• Long gene name
  piwi-interacting RNA cluster 11
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313897
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37518
• Gene
  PIRC14
• Long gene name
  piwi-interacting RNA cluster 14
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313784
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37516
• Gene
  PIRC12
• Long gene name
  piwi-interacting RNA cluster 12
• Locus
  3
• Discovery year
  2009-11-05
• Entrez gene record
  100313808
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

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