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Available ordering format
Lyophilized
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Immunogen
recombinant Arabidopsis thaliana Hcf101, amino acid residues 206-532 Q6STH5, At3g24430
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunolocalization (IL)
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Connected products
AS03 037 | rabbit anti-RbcL stroma marker antibodyPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1:3000 with standard ECL (WB), 1: 3000 (IL)
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Molecular weight expected аpparent
57 | 50.5 kDa
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
Arabidopsis thaliana
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Immunolocalization has been done on isolated plastids, followed by separation into stroma and thylakoids
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References
Bigeard et al. (2014). Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana. Proteomics. 2014 May 31. doi: 10.1002/pmic.201400072.
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Scientific context
Hcf101 is a novel protein required for photosystem I biogenesis. Hcf101-1 is a high-chlorophyll-fluorescence hcf) Arabidopsis thaliana mutant that lacks photosystem I. In green tissue the level of this protein is stimulated by light and the protein has not been detected in roots. It has been localized in chloroplast stroma.
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Notes
cellular [compartment marker] of plastid stroma
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Protein number
Q6STH5
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TAIR number
AT3G24430
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Test
Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
