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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated peptide derived from the sequence of human glycogen phosphorylase P11217
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 50 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunoprecipitation (IP)
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Connected products
AS09 454 | anti-glycogen debranching enzymeSecondary antibodies
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Recommended dilutions for use
1: 2000 (WB), 5 µg (IP)
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Molecular weight expected аpparent
97 kDa
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Verified reactivity
human, mouse, rabbit,rat, Trichomonas vaginalis G3
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Possible reactivity
bovine, dog, hen,horse,pig, Xenopus laevis, Zebrafish, atlantic salmon, Drosophila melanogaster
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
samples used to test this antibody were: muscle and liver homogenates, purified glycogen. This antiboy will recogize glycogen phosphorylase from both liver and muscle. It has been used in ICC on human primary myotubes
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References
Dandanell et al. (2016). Maintaining a clinical weight loss after intensive lifestyle intervention is the key to cardiometabolic health. pii: S1871-403X(16)30107-7. doi: 10.1016/j.orcp.2016.09.009. Bowker and Zhuang (2015). Relationship between water-holding capacity and protein denaturation in broiler breast meat. Poult Sci. 2015 May 25. pii: pev120. Zhu et al. (2011). High post-mortem temperature combined with rapid glycolysis induces phosphorylase denaturation and produces pale and exudative characteristics in broiler Pectoralis major muscles. Meat Scienc
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Scientific context
Glycogen phosphorylase (EC 2.4.1.1) is an enzyme which is catalyzing the rate limiting step in the degradation of glycogen in animals by releasing glucose-1-phosphate from the terminal alpha1,4-glycosidic bond.
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Notes
This antibody can detect GP in crude tissue homogenate in both, liver and muscle tissue and is able to immunoprecipiate GP.Glycogen phosphorylase is a good marker for determining the degree of postmortem protein denaturation in muscle. When glycogen phosphorylase denatures it shifts from the sarcoplasmic fraction of the muscle to the myofibrillar fraction of the muscle. This shift is detectable using SDS-PAGE and western blotting techniques. The antibody is being used to detect glycogen phosphorylase levels in both sarcoplasmic and myofibrillar protein extracts from chicken muscle.
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Protein number
P11217
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TAIR number
Refer to NCBI
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