Goat, Goat anti-biotin, ALP conjugated
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Catalog numberAS10 1452
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Price:
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Size1 mg
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Available ordering formatLiquid
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ImmunogenBiotin
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Raised inGoat
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified IgG
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionNon-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Verified applicationsContact Gentaur support center
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Connected productsother secondary antibodies against biotin
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Recommended dilutions for useThis conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500 - 1:2000 for Immunohistochemistry 1:50 000 - 1:5 000 for ELISA/Western blot
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Molecular weight expected аpparentsee datasheet
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Verified reactivityto be determined
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Possible reactivityto be determined
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No reactivityto be determined
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Supplementary informationContact Gentaur support center at [email protected]
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ReferencesRefer to PubMed
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Scientific contextGoat anti-biotin is a secondary antibody conjugated to ALP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.
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NotesPurity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
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Protein numberRefer to NCBI
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TAIR numberRefer to NCBI
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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Latin nameCapra aegagrus hircus
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Gene target
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Gene symbolPDLIM3, ASRGL1, ATRNL1, SLPI, CCL27
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Short nameGoat, Goat anti- , ALP conjugated
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Techniqueanti-, anti, Goat, antibody to, antibodies, antibody Conjugates, goats
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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LabelBiotin
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SpeciesGoats
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Alternative namecaprine, caprine Antibody tobiotinilated, ALP coupled
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Alternative techniqueantibodies, caprine
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Gene info
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Identity
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Gene
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Long gene namePDZ and LIM domain 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-02-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PDZ domain containing
- LIM domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameasparaginase and isoaspartyl peptidase 1
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Synonyms gene name
- asparaginase like 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-08-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameattractin like 1
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Synonyms gene name
- attractin-like 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-03-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesecretory leukocyte peptidase inhibitor
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Synonyms gene name
- secretory leukocyte protease inhibitor (antileukoproteinase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-24
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Entrez gene record
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Pubmed identfication
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Classification
- WAP four-disulfide core domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 27
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 27
- chemokine (C-C motif) ligand 27
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-07-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data