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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated peptide derived from available glutamyl-tRNA reductase sequences including Arabidopsis thaliana P49294
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
Plant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 5000 with standard ECL (WB)
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Molecular weight expected аpparent
58 | kDa (Arabidopsis thaliana)
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Verified reactivity
Arabidopsis thaliana, Hordeum vulgare
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Possible reactivity
dicots including: Brassica napus, Glycine max, Pisum sativum, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Vitis vinifera, monocots including: Hordeum vulgare, Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa, moss: Physcomitrella patens, algae: Chlamydomonas reinhardtii
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No reactivity
cyanobacteria
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Supplementary information
to be added when available
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References
Nishimura et al. (2013). ClpS1 Is a Conserved Substrate Selector for the Chloroplast Clp Protease System in Arabidopsis. The Plant Cell June 2013.
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Scientific context
GluTR (glutamyl tRNA reductase) belongs to a family of oxidoreductases and is involved in porphyrin and chlorophyll biosynthesis. This enzyme class is called L-glutamate-semialdehyde: NADP+ oxidoreductase (L-glutamyl-tRNAGlu-forming).
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Notes
Antibody reacts with recombinant GluTR isoforms: AtGluTR1, AtGluTR2 and NtGluTR1 (At - Arabidopsis thaliana, Nt - Nicotiana tabacum).
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Protein number
P49294
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TAIR number
AT1G09940
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