Fibrinogen, labelled with fluorescein
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Catalog number
IMS09-038-335
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Price
Please ask
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Size
100 µl (0.2mg/ml)
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Available ordering format
Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
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Immunogen
Purified, full length native fibrinogen Q9UE34
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Raised in
Hen
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified IgY
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
Flow cytometry (FC)
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Connected products
IMS06-038-312 | Fibrinogen | biotinylated antibody
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Recommended dilutions for use
1: 10 (FC)
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Molecular weight expected аpparent
24 kDa
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Verified reactivity
human, porcine, rat, rabbit
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Possible reactivity
bovine, mouse
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
the antibodies have been shown to react with activated human, porcine, rat and rabbit platelets
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References
Terada et al. (2014). Effects of riboflavin and ultraviolet light treatment on platelet thrombus formation on collagen via integrin αIIbβ3 activation. Transfusion. 2014 Feb 8. doi: 10.1111/trf.12566.Schmidt et al. (2012). Phenotyping of Staphylococcus aureus reveals a new virulent ST398 lineage. Clin. Microb. and Infection.
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Scientific context
Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N-terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases. The normal fibrinogen concentration in plasma is about 3 mg/ml. The elevated level of fibrinogen in patient's blood is regarded as an independent risk factor for cardiovascular diseases. An increase in blood fibrinogen concentration was shown to be a strong predictor of coronary heart disease (Sonel et al. 2000; Rapold et al. 1989).
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Notes
The IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation. Labelled with fluorescein. Affinity purified on human fibrinogen agarose.
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Protein number
Q9UE34
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TAIR number
Refer to NCBI
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Gene target
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Short name
Fibrinogen, labelled with fluorescein
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Alternative name
Fibrinogen, labelled including fluorescein
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MeSH Data
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Name
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Concept
Scope note:
Use of written, printed, or graphic materials upon or accompanying a drug container or wrapper. It includes contents, indications, effects, dosages, routes, methods, frequency and duration of administration, warnings, hazards, contraindications, side effects, precautions, and other relevant information.
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Tree numbers
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Qualifiers
trends, ethics, history, classification, economics, instrumentation, methods, standards, legislation & jurisprudence, statistics & numerical data
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