2, Set of 4 Chlamydomonas anti-Lhc antibodies

• Catalog number
  AS01 011 CHLAMYDOMONAS
• Price
  Please ask
• Size
  50 µl (Lhcb5, Lhcbm5), 100 µl (Lhcb4), 200 µl (Lhcb2)

• Available ordering format
  Lyophilized
• Immunogen
  Lhcb2 - BSA-conjugated peptideLhcb4 - KLH-conjugated synthetic peptide derived from Lhcb4 (CP29, Lhcbm4) protein sequence from Chlamydomonas reinhardtii (Q93WD2)Lhcb5 - KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii Lhcb5 protein sequence (Q9FEK6)Lhcbm5 - SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Total IgG in PBS pH 7.4 (Lhcb2), serum (Lhcb4, Lhcb5, Lhcbm5)
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB)
• Connected products
  other antibodies against pigment-binding proteins LHCPlant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1:5000 with standard ECL (WB) (Lhcb2,Lhcb5, Lhcbm5)1: 10 000 with standard ECL (Lhcb4)
• Molecular weight expected аpparent
  25 kDa (Lhcb2)29 kDa (Lhcb4)29 | 30 kDa (Lhcbm5)30| 26 kDa (Lhcb5)
• Verified reactivity
  Chlamydomonas reinhardtii
• Possible reactivity
  angiosperms (monocots and dicots), gymnosperms, mosses, green algae (Lhcb2)
• No reactivity
  Lhcb4 - higher plants (use AS04 045 for those organsims), algae, cyanobacteria
• Supplementary information
  to be added when available
• References
  Lhcb5 and Lhcbm5 - Takahashi et al. (2006). Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii. PNAS 103:477-482
• Scientific context
  The outer light-harvesting antenna of the photosystems (PSI and PSII) of the green unicellular alga Chlamydomonas reinhardtii is composed of pigment-binding proteins belonging to the Lhc family highly conserved in photosynthetic eukaryotes. Some of the Lhc-subtypes of Chlamydomonas share sufficient high similarity with the respective functional homologs in plants to allow specific detection with antisera generated against conserved peptide domains from plant Lhc-proteins.
• Notes
  None
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.

• Gene target
  Set   Chlamydomonas   Lhc   antibodies  
• Short name
  2, Set of 4 Chlamydomonas anti-Lhc antibodies
• Technique
  anti-, anti, antibody to, antibodies
• Alternative name
  2, Set on 4 Chlamydomonas Antibody toLhc antibodies
• Alternative technique
  antibodies
• Tissue
  set

MeSH Data

• Name
  Blood Grouping and Crossmatching
• Concept
  Scope note: Testing erythrocytes to determine presence or absence of blood-group antigens, testing of serum to determine the presence or absence of antibodies to these antigens, and selecting biocompatible blood by crossmatching samples from the donor against samples from the recipient. Crossmatching is performed prior to transfusion.
• Tree numbers
  • E01.370.225.625.120
  • E01.370.225.812.385.120
  • E05.200.625.120
  • E05.200.812.385.120
  • E05.478.594.385.120
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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