Goat IgG F(ab')2 fragment-FITC conjugate
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Catalog number
20011-FAB2-F
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Price
Please ask
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Size
100 tests
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Stock availability
Available
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Category
Isotype Control
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Antibody type
N/A
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Antibody host
N/A
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Antibody conjugate
FITC
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Technical datasheet
Contact Gentaur to request the datasheet or ask our specialists for more information.
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Notes
The Goat IgG F(ab')2 fragment-FITC conjugate is manufactured for Research Use Only or for diagnostics purposes.
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Group
f(ab')2s
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Properties
This adi Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. adi FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Gene symbol
KYAT1, KYAT3, ABCC1
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Short name
Goat IgG F(ab')2 -FITC conjugate
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Technique
F(ab')2 fragment, F(ab')2, FITC, Goat, IgG, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. f(ab')2 fragments, Fluorescein, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, Mouse IgG1 Fab and F(ab')2 Preparation Kit , Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common. IgG
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Label
FITC
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Species
Goat, Goats
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Alternative name
caprine Immunoglobulin G F(Antibody')2 fragment-fluorecein conjugate
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Alternative technique
secondairy, caprine, fluorescine, immunoglobulins
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Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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