Mouse IgM, Clone: DD5, Negative Control, FITC

  • Catalog number
    OBT3289F
  • Price
    Please ask
  • Size
    vial
  • Alternative name 1
    Mouse IgM, Clone: DD5, Negative Control, FITC
  • Alternative name 2
    Mouse IgM, Clone: DD5, Negative Control, FITC
  • Alternative name 3
    Mus musculus IgM, Clone: DD5, Negative Control, FITC
  • Alternative name 4
    anti- IgM Clone: DD5 Negative Control
  • Alternative name 5
    IgM Clone: DD5 Negative Control
  • Clonality
    Monoclonal Antibody
  • Description
    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
  • Storage and handling
    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
  • Advisory
    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
  • Additional description
    Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
  • Properties
    This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
  • Conjugation
    Anti-FITC Antibody
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
    DD5  
  • Gene symbol
    UBR5
  • Short name
    Mouse IgM, Clone: DD5, Negative Control, FITC
  • Technique
    Negative, Control, Mouse, FITC, IgM, controls, Fluorescein, IgMs, mouses, negatif
  • Host
    mouse
  • Isotype
    IgM, IgM
  • Label
    FITC
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Immunoglobulin M, clonality: DD5, Negative reference, fluorecein
  • Alternative technique
    controls, murine, fluorescine, immunoglobulin-m
  • Tissue
    control
Gene info
  • Identity
  • Gene
  • Long gene name
    ubiquitin protein ligase E3 component n-recognin 5
  • Synonyms gene
  • Synonyms gene name
    • E3 ubiquitin protein ligase, HECT domain containing, 1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2005-07-08
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Ubiquitin protein ligase E3 component n-recognins
    • HECT domain containing
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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