Mouse IgG2a, Negative Control for Human, Clone: MRCOX-34 (antibody-Rat), RPE; flow
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Catalog numberOBT0387R
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Price:
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Size100 tests
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Alternative name 1Mouse IgG2a, Negative Control for Human, Clone: MRCOX-34 (anti-Rat), RPE; flow
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Alternative name 2Mouse IgG2a, Negative Control for Human, Clone: MRCOX-34 (antibody-Rat), RPE; flow
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Alternative name 3Mus musculus IgG2a, Negative Control for Human, Clone: MRCOX-34 (antibody-Rattus norvegicustus norvegicus), RPE; flow
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Alternative name 4anti- IgG2a Negative Control for Clone: MRCOX-34 flow
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Alternative name 5IgG2a Negative Control for Clone: MRCOX-34 flow
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional descriptionIsotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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French translationanticorps
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Gene target
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Gene symbolRPE, SNORD115-34, IGKV3D-34, IGHV4-34, IGKV3-34, IGLV2-34, IGHV7-34-1, RNU6-34P, RASSF2, PHF11
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Short nameMouse IgG2a, Negative Control for , Clone: MRCOX-34 (antibody- ), RPE;
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TechniqueAntibody, Negative, Control, Mouse, Flow, antibodies against human proteins, antibodies for, controls, mouses, negatif
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Hostmouse
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IsotypeIgG2a
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Labelrpe
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SpeciesRat, Humans, Mouses, Rats
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Alternative nameMouse IgG2a, Negative reference to measure H. sapiens, clonality: MRCOX-34 ((antibody to-)-Rat), RPE; flow
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Alternative techniquecontrols, antibodies, murine
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Tissuecontrol
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Gene info
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Identity
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Gene
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Long gene nameribulose-5-phosphate-3-epimerase
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-34
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3D-34 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3D-34
- immunoglobulin kappa variable 3D-34 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 4-34
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3-34 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3-34
- immunoglobulin kappa variable 3-34 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 2-34 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable 2-34
- immunoglobulin lambda variable 2-34 pseudogene
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 7-34-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 7-34-1
- immunoglobulin heavy variable 7-34-1 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 34, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 34
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Locus
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Discovery year2008-06-10
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameRas association domain family member 2
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Synonyms gene name
- Ras association (RalGDS/AF-6) domain family member 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ras association domain family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namePHD finger protein 11
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-05-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PHD finger proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
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Tree numbers
- E01.370.225.500.620.670.520
- E01.370.225.750.600.670.520
- E05.200.500.620.670.520
- E05.200.750.600.670.520
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data