Macrophage, Clone: MAC387, Mouse Monoclonal antibody-Human, Baboon, Cat, Dog, Guinea pig, Horse, Monkey, Rabbit, Rat, Swine, Bovine; frozen/paraffin, IH/flow

  • Catalog number
    OBT0371
  • Price
    Please ask
  • Size
    0.25 mg
  • Alternative name 1
    Macrophage, Clone: MAC387, Mab anti-Human, Baboon, Cat, Dog, Guinea pig, Horse, Monkey, Rabbit, Rat, Swine, Bovine; frozen/paraffin, IH/flow
  • Alternative name 2
    Macrophage, Clone: MAC387, Mab antibody-Human, Baboon, Cat, Dog, Guinea pig, Horse, Monkey, Rabbit, Rat, Swine, Bovine; frozen/paraffin, IH/flow
  • Alternative name 3
    Macrophage, Clone: MAC387, Mab antibody-Homo sapiens, Baboon, Cat, Canine, Guinea pig, Horse, Monkey, Rabbit, Rattus norvegicus, Swine, Bovine; frozen/paraffin, IH/flow
  • Alternative name 4
    anti-Macrophage Clone: MAC387 Cat Horse
  • Alternative name 5
    Macrophage Clone: MAC387 Cat Horse
  • Clonality
    Monoclonal Antibody
  • Description
    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
  • Storage and handling
    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
  • Advisory
    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
  • Properties
    If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Most human antibodies and protein are very homologous to baboon genes. BLASTed sequences are used to find homological regions. Felix vulgaris genes or proteins used as antigen, synthetic peptide. Canine or Canis Lupus is mostly Beagle used for drug research. accurate-monoclonals produces ELISA test kits and polyclonal antibodies. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Additional description
    Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular. Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. Often cDNA of monkeys is used as alternative to human cDNA as a model for drug development. Monkeys are generally considered to be intelligent, particularly Old World monkeys.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Pigs and the smaller guinea pigs are frequent used as models for humans. Rabbits are used for polyclonal antibody production by accurate-monoclonals. Rabbit antibodies are very stable and can be stored for several days at room temperature. accurate-monoclonals adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    S100A9
  • Short name
    Macrophage, Clone: MAC387, Mouse Monoclonal antibody- , , Cat, , , , , Rabbit, , Swine, ; frozen/paraffin, IH/
  • Technique
    Mouse monoclonal, Antibody, paraffin, frozen, Rabbit, Mouse, Flow, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG. Rabbits
  • Species
    Bovine, Baboons, Bos Taurus genes are available form the Bovine Genome database. Cats, Dogs, Guinea Pigs, Horses, Humans, Monkeys, Mouses, Pigs and Guinea pigs, Rats
  • Alternative name
    Macrophage, clonality: MAC387, Mouse monoclonal (antibody to-)-H. sapiens, Baboon, catalase, canine, Guinea swine, Horse, Monkey, production species: rabbit, Rat, swine, Bovine; -25°C frozen/paraffin, IH/flow
  • Alternative technique
    antibodies, rabbit-anti, murine
  • Alternative to gene target
    catalase, CAT and IDBG-39262 and ENSG00000121691 and 847, oxidoreductase activity, Plasma membranes, Cat and IDBG-193911 and ENSMUSG00000027187 and 12359, CAT and IDBG-636518 and ENSBTAG00000020980 and 531682
  • Tissue
    frozen
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
  • Tree numbers
    • E01.370.225.500.363.342
    • E01.370.225.500.386.350
    • E05.196.712.516.600.240.350
    • E05.200.500.363.342
    • E05.200.500.386.350
    • E05.242.363.342
    • E05.242.386.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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