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Alternative name 1
Interleukin 6 Receptor (IL-6R), Clone: UV4, Mab anti-Human; flow/ELISA/RIA/dot blot/neutralizes in cell culture
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Alternative name 2
Interleukin 6 Receptor (IL-6R), Clone: UV4, Mab antibody-Human; flow/ELISA/RIA/dot blot/neutralizes in cell culture
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Alternative name 3
Interleukin 6 Receptor (IL-6R), Clone: UV4, Mab antibody-Homo sapiens; flow/ELISA/RIA/dot blot/neutralizes in cell culture
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Alternative name 4
anti-Interleukin 6 Receptor IL-6R Clone: UV4 /RIA/dot blot/neutralizes in cell culture
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Alternative name 5
Interleukin 6 Receptor IL-6R Clone: UV4 /RIA/dot blot/neutralizes in cell culture
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL. Dot blots are simpler than Western blot since no electrophoresis needed. It is advised to use PVDF or Nitrocellulose to deposit the protein samples that will be incubated with the blotting serum before ECL or colorimetric detection. ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Additional description
For cells, cell lines and tissues in culture till half confluency. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels. Radio Immuno Assays are radioactive linked immunosorbent assays that even more than ELISA kits can detect very small levels of antigens. Small proteins, and hormones in blood or serum can be detected by RIAs. The result will be expressed in a XY graphic in which the ration is inverse to the signal.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. RIAs or EIAs or ELISAs for detection and quantification for specific antigenes
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
IL6R, IL6R-AS1, IL36G, IL25, IL22, IL18, IL37, IL17C, IL17A, IL17D
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Short name
Interleukin 6 Receptor (IL-6R), Clone: UV4, Mouse Monoclonal antibody- ; /ELISA/RIA/dot blot/neutralizes in culture
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Technique
Mouse monoclonal, Antibody, Dot Blot, culture, ELISA, Mouse, Blot, Flow, dot, Dot, RIA, antibodies against human proteins, antibodies for, western Blots, cicular dots, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, antigen assay ELISA kit 1 Kit (96 Wells), Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Species
Human, Humans, Mouses
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Alternative name
Interleukin 6 Receptor (Interleukin-6R), clonality: UV4, Mouse monoclonal (antibody to-)-H. sapiens; flow/Enzyme-linked immunosorbent assay/RIA/dot blot/neutralizes in cellular culture
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Alternative technique
antibodies, murine, elisas, western, blots
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Tissue
cell
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