Hematopoietic & non-Hematopoeitic Cell, CD59, MAC Inhibitor, gp18-20, Clone: MEM 43, Mouse Monoclonal antibody-Human, RPE; flow
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Catalog numberOBT0393R
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PricePlease ask
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Sizevial
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Alternative name 1Hematopoietic & non-Hematopoeitic Cell, CD59, MAC Inhibitor, gp18-20, Clone: MEM 43, Mab anti-Human, RPE; flow
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Alternative name 2Hematopoietic & non-Hematopoeitic Cell, CD59, MAC Inhibitor, gp18-20, Clone: MEM 43, Mab antibody-Human, RPE; flow
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Alternative name 3Hematopoietic & non-Hematopoeitic Cell, CD59, MAC Inhibitor, gp18-20, Clone: MEM 43, Mab antibody-Homo sapiens, RPE; flow
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Alternative name 4anti-Hematopoietic & non-Hematopoeitic Cell CD59 MAC Inhibitor gp18-20 Clone: MEM 43 flow
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Alternative name 5Hematopoietic & non-Hematopoeitic Cell CD59 MAC Inhibitor gp18-20 Clone: MEM 43 flow
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional descriptionFor cells, cell lines and tissues in culture till half confluency. Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolRPE, LGALS3, ITGAM, ITGB2, LGALS3BP, CD59
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Short nameHematopoietic & non-Hematopoeitic , CD59, MAC Inhibitor, gp18-20, Clone: MEM 43, Mouse Monoclonal antibody- , RPE;
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TechniqueMouse monoclonal, Antibody, Mouse, Flow, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Labelrpe
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SpeciesHuman, Humans, Mouses
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Alternative nameHematopoietic & non-Hematopoeitic cellular, CD59 molecule, complement regulatory protein, MAC suppressor, gp18-20, clonality: MEM 43, Mouse monoclonal (antibody to-)-H. sapiens, RPE; flow
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Alternative techniqueantibodies, murine
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Alternative to gene targetCD59 molecule, complement regulatory protein, 16.3A5 and 1F5 and EJ16 and EJ30 and EL32 and G344 and HRF-20 and HRF20 and MAC-IP and MACIF and MEM43 and MIC11 and MIN1 and MIN2 and MIN3 and MIRL and MSK21 and p18-20, CD59 and IDBG-38813 and ENSG00000085063 and 966, protein binding, Extracellular, CD59 and IDBG-636427 and ENSBTAG00000002302 and 505574
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene nameribulose-5-phosphate-3-epimerase
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namegalectin 3
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Synonyms gene
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Synonyms gene name
- lectin, galactoside-binding, soluble, 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-09-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- Galectins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameintegrin subunit alpha M
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Synonyms gene
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Synonyms gene name
- integrin, alpha M (complement component receptor 3, alpha; also known as CD11b (p170), macrophage antigen alpha polypeptide)
- integrin, alpha M (complement component 3 receptor 3 subunit)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-08-05
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Entrez gene record
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RefSeq identity
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Classification
- Integrin alpha subunits
- Complement system regulators and receptors
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameintegrin subunit beta 2
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Synonyms gene
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Synonyms gene name
- integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit)
- integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- Integrin beta subunits
- Complement system regulators and receptors
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namegalectin 3 binding protein
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Synonyms gene name
- lectin, galactoside-binding, soluble, 3 binding protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1994-09-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- BTB domain containing
- Scavenger receptor cysteine rich domain containing
- Receptor ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD59 molecule (CD59 blood group)
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Synonyms gene
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Synonyms gene name
- CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344)
- CD59 antigen, complement regulatory protein
- CD59 molecule, complement regulatory protein
- CD59 molecule
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Synonyms
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Blood group antigens
- Complement system regulators and receptors
- CD molecules
- LY6/PLAUR domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data