CD62L, L-Selectin, LECAM-1, Ly 22, Clone: MEL-14, Rat Mouse Monoclonal antibody-Mouse, azide-free; flow/IP/functional assays
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Catalog numberYSRTMCA1259XZ
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PricePlease ask
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Size1 mg
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Alternative name 1CD62L, L-Selectin, LECAM-1, Ly 22, Clone: MEL-14, Rat Mab anti-Mouse, azide-free; flow/IP/functional assays
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Alternative name 2CD62L, L-Selectin, LECAM-1, Ly 22, Clone: MEL-14, Rat Mab antibody-Mouse, azide-free; flow/IP/functional assays
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Alternative name 3CD62L, L-Selectin, LECAM-1, Ly 22, Clone: MEL-14, Rattus norvegicus Mab antibody-Mus musculus, azide-free; flow/IP/functional assays
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Alternative name 4anti-CD62L L-Selectin LECAM-1 Ly 22 Clone: MEL-14 flow/functional assays
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Alternative name 5CD62L L-Selectin LECAM-1 Ly 22 Clone: MEL-14 flow/functional assays
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional descriptionSodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. accurate-monoclonals supplies azide free antibodies on request.
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameMus musculus, Rattus norvegicus
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French translationanticorps
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Gene target
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Gene symbolRAB38, ARMC9, PIRC60, RAB8A, IGHV3OR16-14, TRN-GTT14-1
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Short nameCD62L, L-Selectin, LECAM-1, Ly 22, Clone: MEL-14, Mouse Monoclonal antibody-Mouse, -free; /IP/functional assays
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TechniqueFunctional Assays, Mouse monoclonal, functional, Antibody, Mouse, Flow, IP, antibodies against human proteins, antibodies for, Immunoprecipitation, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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HostRat, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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SpeciesMouse, Mouses, Rats
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Alternative nameCD62L, L-Selectin, LECAM-1, Ly 22, clonality: MEL-14, Rat Mouse monoclonal (antibody to-)-Mouse, azide-free; flow/IP/functional tests
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Alternative techniqueantibodies, murine
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Gene info
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Identity
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Gene
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Long gene nameRAB38, member RAS oncogene family
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-09-16
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Entrez gene record
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Pubmed identfication
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Classification
- RAB, member RAS oncogene GTPases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namearmadillo repeat containing 9
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-10-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo repeat containing
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 60
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nameRAB8A, member RAS oncogene family
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Synonyms gene
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Synonyms gene name
- mel transforming oncogene (derived from cell line NK14)
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Synonyms
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RAB, member RAS oncogene GTPases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR16-14 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3/OR16-14
- immunoglobulin heavy variable 3/OR16-14 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
Gene info
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Identity
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Gene
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Long gene nametRNA-Asn (GTT) 14-1
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Synonyms gene
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Synonyms gene name
- transfer RNA asparagine 4 (anticodon GUU)
- transfer RNA-Asn (GTT) 14-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
MeSH Data
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Name
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ConceptScope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data