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Alternative name 1
CD26, Dipeptidyl Peptidase IV (DPP IV), Adenosine Deaminase (ADA)- Binding Protein, 110kD, Clone: 202-36, Mab anti-Human, Rat, NO X w/Swine or Sheep; paraffin, IH/flow/EM/Affinity purification
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Alternative name 2
CD26, Dipeptidyl Peptidase IV (DPP IV), Adenosine Deaminase (ADA)- Binding Protein, 110kD, Clone: 202-36, Mab antibody-Human, Rat, NO X w/Swine or Sheep; paraffin, IH/flow/EM/Affinity purification
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Alternative name 3
CD26, Dipeptidyl Peptidase IV (DPP IV), Adenosine Deaminase (ADA)- Binding Protein, 110kD, Clone: 202-36, Mab antibody-Homo sapiens, Rattus norvegicus, NO X w/Swine or Ovine; paraffin, IH/flow/EM/Affinity purification
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Alternative name 4
anti-CD26 Di ptidyl ptidase IV DPP IV Adenosine Deaminase ADABinding Protein 110kD Clone: 202-36 NO X w/ or /EM/Affinity purication
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Alternative name 5
CD26 Di ptidyl ptidase IV DPP IV Adenosine Deaminase ADABinding Protein 110kD Clone: 202-36 NO X w/ or /EM/Affinity purication
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Test
A high affinity purification column was use to purify CD26, Dipeptidyl Peptidase IV (DPP IV), Adenosine Deaminase (ADA)- Binding Protein, 110kD, Clone: 202-36, Mouse Monoclonal antibody- , , NO X w/Swine ; paraffin, IH/ /EM/ purification by accurate-monoclonals by chromatographic size exclusion. Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. accurate-monoclonals supplies purification kits and ultra pure reagents.
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Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Mus musculus, Rattus norvegicus
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French translation
anticorps
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Gene target
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Gene symbol
ADA, SNORD115-36, IGKV2D-36, SNORD68, IGHV3-36, IGKV2-36, IGLV1-36, DPP4, MIR202, SNORA35
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Short name
CD26, Dipeptidyl Peptidase IV (DPP IV), Adenosine Deaminase (ADA)- Binding Protein, 110kD, Clone: 202-36, Mouse Monoclonal antibody- , , NO X w/Swine ; paraffin, IH/ /EM/ purification
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Technique
Affinity Purification, Mouse monoclonal, Purification, affinity, Antibody, paraffin, Mouse, Flow, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals, purified
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Species
Sheep, Humans, Mouses, Rats, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
CD26, Dipeptidyl Peptidase IV (DPP IV), Adenosine Deaminase (adenosine deaminase)- Binding Protein, 110kD, clonality: 202-36, Mouse monoclonal (antibody to-)-H. sapiens, Rat, NO X w/swine and ovine; paraffin, IH/flow/EM/protein purification
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Alternative technique
purifications, antibodies, murine
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Alternative to gene target
adenosine deaminase, ADA and IDBG-76830 and ENSG00000196839 and 100, deaminase activity, Extracellular, Ada and IDBG-211962 and ENSMUSG00000017697 and 11486, ADA and IDBG-642808 and ENSBTAG00000005280 and 280712
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