EasyScript Plus Reverse Transcriptase (PCR)

  • Catalog number
    G177
  • Price
    Please ask
  • Size
    25 x 20 ul reactions
  • Product category
    PCR, RT-PCR & qPCR
  • Application
    PCR. For others please contact us
  • Literature
    Refer to NCBI PubMed for current references.
  • Technical datasheet
    Please contact us to receive a datasheet or any other useful technical documentation available.
  • Group
    PCR, polymerase chain reaction
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
  • Properties
    Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
  • Description
    Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
  • Goup
    reverse transcription
  • Gene target
  • Short name
    EasyScript Plus Reverse Transcriptase (PCR)
  • Technique
    reverse, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
  • Alternative name
    EasyScript Plus Reverse Transcriptase (PCR test kit)
  • Alternative technique
    transctription, dna-amplification
MeSH Data
  • Name
  • Concept
    Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
  • Tree numbers
    • E05.393.620
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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