BECN1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

DNA lentivector for transduction information

Lentiveral packaging plasmid DNA for non-viral plasmid transfection and direct use in plasmid expression. This DNA can alos be used for packaging into Lentiviral particles for high efficiency transduction and stably integrated expressions. GENTAUR suggests to use our ABM packaging mix LV003 of second generation virusses or the LV053, our 3rd Generation Packaging mixture. pLenti lentiviral plasmids DNAs are stored in 10milliMolar Tris/HCI with 1mM EDTA at a pH of 8 at -25 C. Vectors with selection markers like kanamycin, puromycin or cumate are available.
Lentiviral technical files
Lentivirus references
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Gene target

BECN1 Lentiviral Vector CMV pLenti-GIII-CMV-RFP-2A-Puro
Gene symbol

TRIM27, IRX5, PLA2G3, BECN1, IGHD2OR15-2A, EIF2A, CXCL2, RNVU1-2A, PLPP1, CHD3
Short name

BECN1 Lentiviral Vector ( ) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
Technique

Vectors
Label

puro
Species

Human, Humans
Alternative name

beclin 1, autophagy related Lentiviral integrating Desoxyribonucleic acid sequence (H. sapiens) (cytomegalovirus) (pLenti-GIII-cytomegalovirus-Red Fluorescent-2A-Puro)
Alternative to gene target

beclin 1, autophagy related, ATG6 and beclin1 and VPS30, BECN1 and IDBG-51769 and ENSG00000126581 and 102725513,8678, sequence-specific DNA binding, Plasma membranes, Becn1 and IDBG-212053 and ENSMUSG00000035086 and 56208, BECN1 and IDBG-640456 and ENSBTAG00000019914 and 527278
Virus

cmv

Identity

HGNC:1034
Gene

BECN1
Long gene name

beclin 1
Synonyms gene name
- beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
- beclin 1, autophagy related
Synonyms
- ATG6
- VPS30
Synonyms name
- ATG6 autophagy related 6 homolog (S. cerevisiae)
GenBank acession
- AF077301
Locus

17q21.31
Discovery year

1998-11-27
Entrez gene record

8678
Pubmed identfication
- 9765397
RefSeq identity
- NM_003766
Classification
- PIK3C3 complex subunits
- MicroRNA protein coding host genes
- Autophagy related
VEGA ID

OTTHUMG00000180653

Identity

HGNC:1918
Gene

CHD3
Long gene name

chromodomain helicase DNA binding protein 3
Synonyms
- Mi-2a
- ZFH
- Mi2-ALPHA
GenBank acession
- U08379
Locus

17p13.1
Discovery year

1998-03-20
Entrez gene record

1107
Pubmed identfication
- 9326634
- 7560064
RefSeq identity
- NM_001005273
Classification
- Small nucleolar RNA protein coding host genes
- NuRD complex subunits
- PHD finger proteins
VEGA ID

OTTHUMG00000150427

Name

Artificial Gene Fusion
Concept

Scope note: The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.
Tree numbers
- E05.393.220.250
Qualifiers

drug effects, methods, radiation effects