FITC Donkey Anti Rabbit IgG (H+L) Secondary Antibody

• Catalog number
  AS042
• Price
  Please ask
• Size
  100ul

• Antibody category
  Secondary Antibody
• Gene name
  IgG
• Clonality
  Secondary Antibody
• Reactivity
  none
• Conjugate
  FITC
• Applications
  Immunofluorescence, Flow cytometry
• Ordering
  To order the FITC Donkey Anti Rabbit IgG (H+L) Secondary Antibody (Cat. ID#AS042), please submit a purchase order by fax or e-mail. Please contact us for bulk or larger quantity.
• Tips
  Please follow carefully the instructions before every use. Check the vial for the expiry date and batch number. If you need technical assistance please do not hesitate to contact us.
• Notes
  For Research Use Only, not for therapeutic or diagnostic purposes. Not for human or animal consumption.
• Datasheet
  Please contact our technical support to request the latest datasheet, protocol, certificate of analysis, MSDS.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by ABclonal they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This ABclonal Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. ABclonal FITC is highly sensitive to pH extremes.
• Latin name
  Equus africanus asinus, Oryctolagus cuniculus
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by ABclonal. Rabbit antibodies are very stable and can be stored for several days at room temperature. ABclonal adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• French translation
  anticorps

• Gene target
  H+L   Secondary  
• Short name
  FITC Anti Rabbit IgG (H+L) Secondary Antibody
• Technique
  Antibody, Rabbit, anti, FITC, IgG, antibody to, antibodies against human proteins, antibodies for, Fluorescein, IgGs
• Host
  Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. Rabbits
• Isotype
  IgG, IgG
• Label
  FITC
• Species
  Donkeys
• Alternative name
  fluorecein Donkey antibody to production species: rabbit Immunoglobulin G (heavy + light chain) secondary detector Antibody (antibody to-)
• Alternative technique
  antibodies, rabbit-anti, fluorescine, immunoglobulins

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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