p53 Antibody

• Catalog number
  F52116-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  p53
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, ELISA
• Recommended dilutions
  Western blot: 1:1000
• Notes
  Titration of the p53 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This p53 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P04637
• Purity
  Antigen affinity
• Description
  Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
• Immunogen
  A portion of amino acids 187-215 from the human protein was used as the immunogen for this p53 antibody.
• Storage
  Aliquot the p53 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  p53  
• Gene symbol
  MDM4, TP53, LNCPRESS2
• Short name
  Anti-p53
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to p53
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6974
• Gene
  MDM4
• Long gene name
  MDM4 regulator of p53
• Synonyms gene name
  • mouse double minute 4, human homolog of; p53-binding protein
  • Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
  • Mdm4 p53 binding protein homolog (mouse)
  • MDM4, p53 regulator
• Synonyms
  • MDMX
  • HDMX
• GenBank acession
  • AF007111
• Locus
  1q32.1
• Discovery year
  1997-09-05
• Entrez gene record
  4194
• Pubmed identfication
  • 9226370
  • 14660608
  • 26798135
• RefSeq identity
  • NM_002393
• Classification
  • Ring finger proteins
• VEGA ID
  OTTHUMG00000035877

Gene info

• Identity
  HGNC:11998
• Gene
  TP53
• Long gene name
  tumor protein p53
• Synonyms
  • p53
  • LFS1
• Synonyms name
  • Li-Fraumeni syndrome
• GenBank acession
  • AF307851
• Locus
  17p13.1
• Discovery year
  1986-01-01
• Entrez gene record
  7157
• Pubmed identfication
  • 6396087
  • 3456488
  • 2047879
• RefSeq identity
  • NM_000546
• VEGA ID
  OTTHUMG00000162125
• Locus Specific Databases
  • IARC TP53 Mutation Database
  • p53 UMD TP53 mutation database
  • Database of Germline p53 Mutations
  • MUTP53LOAD, Mutant p53 Loss Of Activity Database
  • LRG_321

Gene info

• Identity
  HGNC:52747
• Gene
  LNCPRESS2
• Long gene name
  lncRNA p53 regulated and ESC associated 2
• Synonyms name
  • lncRNA p53 regulated and ESC specific 2
• Locus
  4q22.1
• Discovery year
  2016-12-14
• Entrez gene record
  101929194
• Pubmed identfication
  • 27912097
• RefSeq identity
  • NR_125920
• Classification
  • Long non-coding RNAs with non-systematic symbols
• VEGA ID
  OTTHUMG00000160917

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products