p53 Antibody
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Catalog numberV3343-20UG
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PricePlease ask
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Size20 ug
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CategoryAntibody
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Concentration0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
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FormPurified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenp53
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens). Others not known. ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsFACS, IF, WB, IHC-P
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Recommended dilutionsFlow Cytometry: 0.5-1ug/million cells in 0.1ml,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 minutes at RT
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NotesThe optimal dilution of the p53 antibody for each application should be determined by the researcher.
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Intented useThis p53 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotP04637
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PurityPurified by protein A Affinity Chromatography
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DescriptionRecognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
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ImmunogenRecombinant human p53 protein was used as the immunogen for this p53 antibody.
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StorageStore the p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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LocalizationPredominantly nuclear
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMDM4, TP53, LNCPRESS2
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Short nameAnti-p53
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to p53
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameMDM4 regulator of p53
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Synonyms gene name
- mouse double minute 4, human homolog of; p53-binding protein
- Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
- Mdm4 p53 binding protein homolog (mouse)
- MDM4, p53 regulator
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-09-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ring finger proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametumor protein p53
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namelncRNA p53 regulated and ESC associated 2
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Synonyms name
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Locus
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Discovery year2016-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long non-coding RNAs with non-systematic symbols
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data