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Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr180 and Tyr182 of human p38a (AA177-182)
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Antibody s target
Human p38a (pThr180+pTyr182)
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Antibody s full description
Rabbit Anti-Human p38a (pThr180+pTyr182) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
CSAID-binding protein antibody, Csaids binding protein antibody, CSBP 1 antibody, CSBP 2 antibody, Cytokine suppressive anti-inflammatory drug-binding protein antibody, EXIP antibody, MAP kinase 14 antibody, MAP kinase MXI2 antibody, MAP kinase p38 alpha antibody, MAPK 14 antibody, MAX interacting protein 2 antibody, Mitogen Activated Protein Kinase 14 antibody, Mitogen activated protein kinase p38 alpha antibody, Mitogen-activated protein kinase 14 antibody, Mitogen-activated protein kinase p38 alpha antibody, MK14_HUMAN antibody, Mxi 2 antibody, p38 ALPHA antibody, p38 antibody, p38 MAP kinase antibody, p38 MAPK antibody, p38 mitogen activated protein kinase antibody, p38ALPHA antibody, p38alpha Exip antibody, PRKM14 antibody, SAPK2A antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 41.293 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1037 was sufficient for detection of p38a (pThr180+pTyr182) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney
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Scientific context
MAPK14 is a protein-serine/threonine kinase that is activated by pro-inflammatory cytokines, environmental stress and LPS, via phosphorylation by MAP kinase kinases, or autophosphorylation. Its substrates include the transcription regulators ATF2, MEF2C, and MAX; the cell cycle regulator CDC25B; and p53, which indicates that MAPK14 plays a role in stress-related transcription and in cell cycle regulation, as well as in the genotoxic stress response. It is a key player in the maintenance of hematopoiesis homeostasis, as it balances both proliferative and growth inhibitory signals from growth factors/cytokines that regulate hematopoiesis. Alterations in this controlled balance could result in either overproduction or depletion of myelosuppressive cytokines, which could lead to the development of various types of bone marrow failures.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_620581
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Gene number
1432
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Protein number
Q16539
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
