PD-L1 /CD274, Human Recombinant
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Catalog numberP1023-50
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PricePlease ask
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Size50 µg
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SynonymsPD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
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Alternative_namesPD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥80%
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AssaySDS-PAGE
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Purity≥80%
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Biological activityMeasured for its ability to bind Human PD-1 in a functional ELISA with a linear range of 0.125-1 µg/ml.
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ResultsMeasured for its ability to bind Human PD-1 in a functional ELISA with a linear range of 0.125-1 µg/ml.
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Binding CapacityHuman Recombinant PD-L1 when immobilized at 2 µg/ml in a functional ELISA is able to bind biotinylated Human Recombinant PD-1.
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Molecular Weight28.3 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 0.22 um filtered solution in PBS.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background InformationProgrammed death 1 ligand 1 (PD-L1) also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 is involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCD274, ENPP2, MTA2, MICALL1, IGKV1-16, ZBTB4, UCHL1, COLEC10, PDCD1, FAM71F1
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Short namePD-L1 /CD274, Recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative namePD-L1 /CD274 molecule, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetCD274 molecule, CD274 and IDBG-47487 and ENSG00000120217 and 29126, protein binding, Cell surfaces, Cd274 and IDBG-157249 and ENSMUSG00000016496 and 60533, PD-L1 and IDBG-631351 and ENSBTAG00000000095 and 533834
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Gene info
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Identity
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Gene
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Long gene nameCD274 molecule
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Synonyms gene
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Synonyms gene name
- programmed cell death 1 ligand 1
- CD274 antigen
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- C2-set domain containing
- V-set domain containing
- B7 family
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameectonucleotide pyrophosphatase/phosphodiesterase 2
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-08-10
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Entrez gene record
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Pubmed identfication
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Classification
- Ectonucleotide pyrophosphatase/phosphodiesterase family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemetastasis associated 1 family member 2
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Synonyms gene
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Synonyms gene name
- metastasis associated gene family, member 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-05-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- GATA zinc finger domain containing
- NuRD complex subunits
- Myb/SANT domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameMICAL like 1
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Synonyms gene name
- MICAL-like 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-11-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- LIM domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1-16
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namezinc finger and BTB domain containing 4
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- BTB domain containing
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin C-terminal hydrolase L1
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Synonyms gene
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Synonyms gene name
- ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin C-terminal hydrolases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecollectin subfamily member 10
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Synonyms gene name
- collectin sub-family member 10 (C-type lectin)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-11
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Entrez gene record
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Pubmed identfication
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Classification
- Collectins
- C-type lectin domain containing
- Complement system activation components
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprogrammed cell death 1
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Synonyms gene
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Synonyms gene name
- systemic lupus erythematosus susceptibility 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-05-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefamily with sequence similarity 71 member F1
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Synonyms gene
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Synonyms gene name
- family with sequence similarity 137, member A
- family with sequence similarity 71, member F1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2007-07-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data