PD-L1 /CD274, Human Recombinant

• Catalog number
  P1023-50
• Price
  Please ask
• Size
  50 µg

• Synonyms
  PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
• Alternative_names
  PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥80%
• Assay
  SDS-PAGE
• Purity
  ≥80%
• Biological activity
  Measured for its ability to bind Human PD-1 in a functional ELISA with a linear range of 0.125-1 µg/ml.
• Results
  Measured for its ability to bind Human PD-1 in a functional ELISA with a linear range of 0.125-1 µg/ml.
• Binding Capacity
  Human Recombinant PD-L1 when immobilized at 2 µg/ml in a functional ELISA is able to bind biotinylated Human Recombinant PD-1.
• Molecular Weight
  28.3 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from 0.22 um filtered solution in PBS.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
• Background Information
  Programmed death 1 ligand 1 (PD-L1) also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 is involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PD-L1   CD274  
• Gene symbol
  CD274, ENPP2, MTA2, MICALL1, IGKV1-16, ZBTB4, UCHL1, COLEC10, PDCD1, FAM71F1
• Short name
  PD-L1 /CD274, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  PD-L1 /CD274 molecule, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  CD274 molecule, CD274 and IDBG-47487 and ENSG00000120217 and 29126, protein binding, Cell surfaces, Cd274 and IDBG-157249 and ENSMUSG00000016496 and 60533, PD-L1 and IDBG-631351 and ENSBTAG00000000095 and 533834

Gene info

• Identity
  HGNC:17635
• Gene
  CD274
• Long gene name
  CD274 molecule
• Synonyms gene
  • PDCD1LG1
• Synonyms gene name
  • programmed cell death 1 ligand 1
  • CD274 antigen
• Synonyms
  • B7-H
  • B7H1
  • PD-L1
  • PDL1
  • B7-H1
• Synonyms name
  • B7 homolog 1
• GenBank acession
  • AF177937
• Locus
  9p24.1
• Discovery year
  2003-11-13
• Entrez gene record
  29126
• Pubmed identfication
  • 11015443
  • 10581077
• RefSeq identity
  • NM_014143
• Classification
  • Receptor ligands
  • C2-set domain containing
  • V-set domain containing
  • B7 family
  • CD molecules
• VEGA ID
  OTTHUMG00000019503

Gene info

• Identity
  HGNC:3357
• Gene
  ENPP2
• Long gene name
  ectonucleotide pyrophosphatase/phosphodiesterase 2
• Synonyms gene
  • PDNP2
• Synonyms
  • ATX
  • PD-IALPHA
• Synonyms name
  • autotaxin
• GenBank acession
  • D45421
• Locus
  8q24.12
• Discovery year
  1995-08-10
• Entrez gene record
  5168
• Pubmed identfication
  • 8586446
• Classification
  • Ectonucleotide pyrophosphatase/phosphodiesterase family
• VEGA ID
  OTTHUMG00000164995

Gene info

• Identity
  HGNC:7411
• Gene
  MTA2
• Long gene name
  metastasis associated 1 family member 2
• Synonyms gene
  • MTA1L1
• Synonyms gene name
  • metastasis associated gene family, member 2
• Synonyms
  • MTA1-L1
• GenBank acession
  • AB016591
• Locus
  11q12.3
• Discovery year
  1999-05-17
• Entrez gene record
  9219
• Pubmed identfication
  • 9929979
• RefSeq identity
  • NM_004739
• Classification
  • GATA zinc finger domain containing
  • NuRD complex subunits
  • Myb/SANT domain containing
• VEGA ID
  OTTHUMG00000167684

Gene info

• Identity
  HGNC:29804
• Gene
  MICALL1
• Long gene name
  MICAL like 1
• Synonyms gene name
  • MICAL-like 1
• Synonyms
  • MIRAB13
  • KIAA1668
  • MICAL-L1
• Synonyms name
  • molecule interacting with Rab13
• GenBank acession
  • BK000466
• Locus
  22q13.1
• Discovery year
  2006-11-24
• Entrez gene record
  85377
• Pubmed identfication
  • 11258795
  • 12110185
• RefSeq identity
  • NM_033386
• Classification
  • LIM domain containing
• VEGA ID
  OTTHUMG00000150670

Gene info

• Identity
  HGNC:5732
• Gene
  IGKV1-16
• Long gene name
  immunoglobulin kappa variable 1-16
• Synonyms
  • IGKV116
  • L1
• GenBank acession
  • J00248
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28938
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151649

Gene info

• Identity
  HGNC:23847
• Gene
  ZBTB4
• Long gene name
  zinc finger and BTB domain containing 4
• Synonyms
  • KIAA1538
  • KAISO-L1
  • ZNF903
• GenBank acession
  • AB040971
• Locus
  17p13.1
• Discovery year
  2003-12-15
• Entrez gene record
  57659
• Pubmed identfication
  • 16354688
  • 25499219
• RefSeq identity
  • NM_020899
• Classification
  • BTB domain containing
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000108137

Gene info

• Identity
  HGNC:12513
• Gene
  UCHL1
• Long gene name
  ubiquitin C-terminal hydrolase L1
• Synonyms gene
  • PARK5
• Synonyms gene name
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Synonyms
  • PGP9.5
  • Uch-L1
• Synonyms name
  • ubiquitin thiolesterase
• GenBank acession
  • BC000332
• Locus
  4p13
• Discovery year
  1991-07-15
• Entrez gene record
  7345
• Pubmed identfication
  • 1840236
• RefSeq identity
  • NM_004181
• Classification
  • Ubiquitin C-terminal hydrolases
• VEGA ID
  OTTHUMG00000099377

Gene info

• Identity
  HGNC:2220
• Gene
  COLEC10
• Long gene name
  collectin subfamily member 10
• Synonyms gene name
  • collectin sub-family member 10 (C-type lectin)
• Synonyms
  • CL-L1
  • CL-10
• GenBank acession
  • AB002631
• Locus
  8q24.12
• Discovery year
  2000-04-11
• Entrez gene record
  10584
• Pubmed identfication
  • 10224141
• Classification
  • Collectins
  • C-type lectin domain containing
  • Complement system activation components
• VEGA ID
  OTTHUMG00000164971

Gene info

• Identity
  HGNC:8760
• Gene
  PDCD1
• Long gene name
  programmed cell death 1
• Synonyms gene
  • SLEB2
• Synonyms gene name
  • systemic lupus erythematosus susceptibility 2
• Synonyms
  • CD279
  • PD1
  • hSLE1
  • PD-1
• GenBank acession
  • AY206416
• Locus
  2q37.3
• Discovery year
  1994-05-24
• Entrez gene record
  5133
• Pubmed identfication
  • 7851902
  • 12402038
• RefSeq identity
  • NM_005018
• Classification
  • V-set domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000151342

Gene info

• Identity
  HGNC:30704
• Gene
  FAM71F1
• Long gene name
  family with sequence similarity 71 member F1
• Synonyms gene
  • FAM137A
• Synonyms gene name
  • family with sequence similarity 137, member A
  • family with sequence similarity 71, member F1
• Synonyms
  • NYD-SP18
  • GARI-L1
• Synonyms name
  • Golgi-associated RAB2B interactor like 1
• GenBank acession
  • AF367470
• Locus
  7q32.1
• Discovery year
  2007-07-24
• Entrez gene record
  84691
• Pubmed identfication
  • 12477932
  • 34714330
• RefSeq identity
  • NM_032599
• VEGA ID
  OTTHUMG00000158276

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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