Recombinant Human Isocitrate Dehydrogenase 1/IDH1 (C-6His)

• Catalog number
  C943-50
• Price
  Please ask
• Size
  50 ug

• Description
  Recombinant Human Isocitrate Dehydrogenase is produced by our Mammalian expression system and the target gene encoding Met1-Leu414 is expressed with a 6His tag at the C-terminus.
• Species reactivity
  Human
• Origin
  Human cells
• Peptide sequence
  MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKLVDHHHHHH
• Estimated molecular weight
  47,7 kDa
• Protein purity
  Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxin level
  Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Shipping condition
  Dry ice/ice packs
• Package form
  Supplied as a 0.2 µm filtered solution of PBS.
• Storage conditions
  Store at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
• Reconstitution conditions
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• UniProt number
  O75874
• Other related products
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Isocitrate   Dehydrogenase   1/IDH1   C-6His  
• Gene symbol
  IDH1, IDH1-AS1
• Short name
  Recombinant Isocitrate Dehydrogenase 1/IDH1 (C-6His)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Human Isocitrate Dehydrogenase/IDH1(C-6His)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:5382
• Gene
  IDH1
• Long gene name
  isocitrate dehydrogenase (NADP(+)) 1
• Synonyms gene name
  • isocitrate dehydrogenase 1 (NADP+), soluble
  • isocitrate dehydrogenase 1 (NADP+)
  • isocitrate dehydrogenase (NADP(+)) 1, cytosolic
• Locus
  2q34
• Discovery year
  2001-06-22
• Entrez gene record
  3417
• RefSeq identity
  • NM_005896
• Classification
  • Isocitrate dehydrogenases
• VEGA ID
  OTTHUMG00000132943
• Locus Specific Databases
  • LRG_610

Gene info

• Identity
  HGNC:40292
• Gene
  IDH1-AS1
• Long gene name
  IDH1 antisense RNA 1
• Synonyms gene name
  • IDH1 antisense RNA 1 (non-protein coding)
• GenBank acession
  • AI492612
  • BE465811
• Locus
  2q34
• Discovery year
  2012-02-24
• Entrez gene record
  100507475
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000154707

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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