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Synonyms
CD73, NT5E, 5'-Nucleotidase, 5'-NT, NT5, NTE
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Alternative_names
CD73, NT5E, 5'-Nucleotidase, 5'-NT, NT5, NTE
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Description
Enzyme that convert AMP to adenosine
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Recombinant
1
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Source
HEK293
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Purity by SDS PAGE
>85% as determined by SDS-PAGE.
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Endotoxin Level
Less than 1.0 EU per ug by the LAL method.
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Molecular Weight
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.9 kDa. The protein migrates as 30 kDa, 40 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile deionized water, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. It is recommended to store at -20°C.
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Background Information
5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
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Amino acid sequence
AA Trp 27 - Ser 549
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NT5E
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Short name
CellExp™ CD73 / NT5E, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ CD73 / 5'-nucleotidase, ecto (CD73), H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
5'-nucleotidase, ecto (CD73), CALJA and CD73 and E5NT and eN and eNT and NT and NT5 and NTE, NT5E and IDBG-93979 and ENSG00000135318 and 4907, hydrolase activity, Plasma membranes, Nt5e and IDBG-190005 and ENSMUSG00000032420 and 23959
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