Apoptotic Cell Isolation Kit

• Catalog number
  K258-30
• Price
  Please ask
• Size
  30 isolations

• Description
  Isolates apoptotic cells using magnetic beads
• Summary
  • Detection method- Magnetic bead bound apoptotic cells can be separated from normal cells • Species reactivity- Mammalian • Applications- Kit provides a simple and efficient means for isolation of apoptotic cells from normal culture using annexin V magnetic beads (MagBeads). The separated apoptotic cells and normal cells can be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from normal culture.
• Sample Type
  Cell cultures (adherent and suspension)
• Features Benefits
  • Simple procedure; takes only ~1 hours • Fast and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  The Apoptotic Cell Isolation Kit provides a simple and efficient means for isolation of apoptotic cells or removal of dead cells from cell culture or tissue preparations using annexin V/magnetic beads (MagBeads). Annexin V is a Ca²ᶧ-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), which is redistributed from the inner to the outer plasma membrane leaflet in apoptotic or dead cells. Once on the cell surface, PS becomes available for binding to annexin V and any of its conjugates. Binding of annexin V-biotin to apoptotic cells followed by binding of the biotin to streptavidin-MagBeads enables separation of apoptotic cells from living cells. The apoptotic cells bound to the MagBeads adhere to the magnet, while nonapoptotic cells stay in suspension. The separated apoptotic and healthy cells can then be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from healthy cell culture.
• Data Sheet Link
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Apoptotic   Isolation   Kit  
• Short name
  Apoptotic Isolation Kit
• Alternative name
  Apoptotic cellular purification reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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