anti-ADD2 Antibody

  • Catalog number
    26-644
  • Price
    Please ask
  • Size
    50 ug
  • Gene info
    Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known.
  • Gene names
    ADD2, ADDB
  • Antibodie s label
    unlabelled serum
  • Purity
    Antibody is purified by peptide affinity chromatography method.
  • Ab raised in
    Rabbit
  • Type
    Polyclonal
  • Antigen
    Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ADD2.
  • Immuno application
    ELISA, Western Blot
  • Antibody advice
    ADD2 antibody can be used for detection of ADD2 by ELISA at 1:312500. ADD2 antibody can be used for detection of ADD2 by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
  • Suspention pH azide
    Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
  • Keep at
    For short periods of storage (days) store at 4 grades C. For longer periods of storage, store ADD2 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
  • Works with
    Human
  • Omim nr
    9257192
  • Ncbi nr
    NP_001608
  • KDa
    80 kDa
  • Vial with antibody
    Lyophilized
  • Quantity per ml
    1 mg/ml
  • NCBI GENE nr
    119
  • GENE symbol
    ADD2
  • Antigene name
    adducin 2 (beta)
  • Source
    Homo sapiens
  • Protein nr
    P35612
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    ADD2  
  • Gene symbol
    ADD2
  • Short name
    anti-ADD2 Antibody
  • Technique
    Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
  • Alternative name
    Antibody toADD2 (antibody to-)
  • Alternative technique
    antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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