-
Alternative Name
cRaf, RAF1, c Raf, C-raf, C-Raf proto-oncogene, serine/threonine kinase, CMD1NN, Craf 1 transforming gene, cRaf, Craf1 transforming gene, EC 2.7.11.1, kinase Raf1, Murine sarcoma 3611 oncogene 1, NS5, Oncogene MIL, Oncogene RAF1, OTTHUMP00000160218, OTTHUMP00000207813, OTTHUMP00000209389, Protein kinase raf 1, Proto-oncogene c-RAF, Raf 1, Raf 1 proto oncogene serine/threonine kinase, RAF, Raf proto oncogene serine/threonine protein kinase, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1, RAF1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV, v raf 1 murine leukemia viral oncogene homolog 1, v-raf murine sarcoma viral oncogene homolog 1, v-raf-1 murine leukemia viral oncogene-like protein 1, vraf1 murine leukemia viral oncogene homolog 1,
-
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser259 of human Raf1
-
Swiss Prot
P04049(Human) Q99N57(Mouse) P11345(Rat)
-
Reactivity
Human, Mouse, Rat
-
Applications
WB IHC
-
Application_all
WB:1:1,000-1:2,000 IHC:1:50-1:200
-
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
-
Purification Purity
ProA affinity purified
-
Storage Stability
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Specificity
Raf1 (Phospho-S259) polyclonal antibody detects endogenous levels of Raf1 protein only when phosphorylated at S259.
-
Usage
Western blot analysis of Phospho-Raf1(S259) on different lysates using anti-Phospho-Raf1(S259) antibody at 1/1,000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: Hela
-
BiowMW
73 kDa
-
Note
For research use only, not for use in diagnostic procedure.
-
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
French translation
anticorps
-
Gene target
-
Short name
Raf1 (Phospho-S259) polyclonal antibody
-
Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit
-
Alternative name
v-raf-1 murine leukemia viral oncogene homolog 1 (phosphorilated-S259) polyclonal (antibody to-)
-
Alternative technique
polyclonals, antibodies
-
Alternative to gene target
v-raf-1 murine leukemia viral oncogene homolog 1, c-Raf and CMD1NN and CRAF and NS5 and Raf-1, RAF1 and IDBG-19277 and ENSG00000132155 and 102724915,5894, transferase activity, nuclei, Raf1 and IDBG-179428 and ENSMUSG00000000441 and 110157, RAF1 and IDBG-646381 and ENSBTAG00000004514 and 521196
-