IGF-BP1, human recombinant

  • Catalog number
    4717-25
  • Price
    Please ask
  • Size
    25 ug
  • Synonyms
    IBP-1, IGF-Binding Protein 1, AFBP, PP12, IGF-BP25, hIGFBP-1, IGFBP-1.
  • Alternative_names
    IBP-1, IGF-Binding Protein 1, AFBP, PP12, IGF-BP25, hIGFBP-1, IGFBP-1.
  • Description
    A protein which binds and prolongs the half-life of the IGFs
  • Recombinant
    Yes
  • Source
    Nso cells
  • Purity by SDS PAGE
    ≥98%
  • Assay
    SDS-PAGE
  • Purity
    ≥98%
  • Biological activity
    The ED₅₀ as determined by its ability to inhibit IGF-I induced proliferation of NUC-1 cells is ~100 ng/ml.
  • Results
    ~100 ng/ml
  • Molecular Weight
    28.8 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized protein
  • Physical form description
    Sterile filtered and lyophilized from 10 mM HCl.
  • Reconstitution Instructions
    Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.
  • Background Information
    Human IGF-BP1 is a 25.4 kDa cysteine-rich secreted protein expressed in liver, decidual, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, whereas nonphosphorylated IGF-BP1 is stimulatory. Recombinant human IGF-BP1 is a single, glycosylated protein consisting of 218 amino acid residues.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional description
    IGF-I, IGF-II, insulin-like growth factors -1 and -2 are important regulators of signaling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    BP1  
  • Gene symbol
    SIGMAR1, EIF4EBP1, REXO1, DLX4, GORAB, ATF2, NAE1, TSPOAP1, SIGLEC6
  • Short name
    IGF-BP1, recombinant
  • Technique
    Recombinant, IGF, igfs, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    IGF-BP1, H. sapiens Rec.
  • Alternative technique
    rec, igfs
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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