Nucleolin Antibody

• Catalog number
  F41619-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Nucleolin
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC, IF, ELISA
• Recommended dilutions
  Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
• Notes
  The stated application concentrations are suggested starting amounts. Due to differences in protocols and secondary/substrate sensitivity, it may be necessary to titrate the Nucleolin antibody for optimal performance.
• Intented use
  This Nucleolin antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P19338
• Purity
  Antigen affinity
• Description
  Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [Wiki]
• Immunogen
  The amino acid sequence used as immunogen for this Nucleolin antibody is found between 425-455 of the human protein.
• Storage
  The Nucleolin antibody should be aliquoted and and stored frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeat freeze-thaws.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Nucleolin  
• Gene symbol
  NCL, NCLP1, NCLP2
• Short name
  Anti-Nucleolin
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to Nucleolin
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:7667
• Gene
  NCL
• Long gene name
  nucleolin
• Synonyms
  • C23
  • Nsr1
• Locus
  2q37.1
• Discovery year
  1991-01-15
• Entrez gene record
  4691
• Pubmed identfication
  • 2394707
  • 3409881
• RefSeq identity
  • NM_005381
• Classification
  • Small nucleolar RNA protein coding host genes
  • SSU processome
  • DNA helicases
  • RNA binding motif containing
• VEGA ID
  OTTHUMG00000153866

Gene info

• Identity
  HGNC:35472
• Gene
  NCLP1
• Long gene name
  nucleolin pseudogene 1
• Locus
  9q34.3
• Discovery year
  2009-03-03
• Entrez gene record
  100302751
• RefSeq identity
  • NG_012793
• VEGA ID
  OTTHUMG00000020939

Gene info

• Identity
  HGNC:35473
• Gene
  NCLP2
• Long gene name
  nucleolin pseudogene 2
• Synonyms
  • bA85L21.1
• Locus
  Xq26.3
• Discovery year
  2009-03-03
• Entrez gene record
  100379142
• RefSeq identity
  • NG_016573
• VEGA ID
  OTTHUMG00000022467

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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