-
Synonyms
ERBB3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3
-
Alternative_names
ERBB3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3
-
Description
Involved in pathways leading to cell proliferation or differentiation.
-
Recombinant
True
-
Source
HEK 293 cells
-
Purity by SDS PAGE
>90%
-
Endotoxin Level
Less than 1.0 EU per ug by the LAL method.
-
Activity Specifications test method
Immobilized Human NRG1-beta1 Protein (ECD, Fc Tag) at 2 ug/mL (100 uL/well) can bind Human ErbB3, Mouse IgG2a Fc Tag with a linear range of 0.3-2 ng/mL
-
Molecular Weight
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 95.5 kDa. The protein migrates as 115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
-
Storage Temp
-20ºC
-
Shipping
Gel Pack
-
Shelf Life
12 months
-
Appearance
Lyophilized powder
-
Physical form description
Lyophilized
-
Reconstitution Instructions
Reconstitute in sterile deionized water to the desired protein concentration.
-
Background Information
ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
-
Amino acid sequence
Ser 20 - Thr 643
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Short name
Cellxp™ Her3 / ErbB3, Fc Tag, Recombinant
-
Technique
Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
-
Label
Fc Tag
-
Species
Humans
-
Alternative name
H. sapiens Cellxp™ Her3 / v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian), fragment c detection labelled, H. sapiens Rec.
-
Alternative technique
rec
-
Alternative to gene target
v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian), c-erbB-3 and c-erbB3 and ErbB-3 and erbB3-S and HER3 and LCCS2 and MDA-BF-1 and p180-ErbB3 and p45-sErbB3 and p85-sErbB3, ERBB3 and IDBG-39824 and ENSG00000065361 and 2065, transferase activity, Extracellular, Erbb3 and IDBG-197943 and ENSMUSG00000018166 and 13867, ERBB3 and IDBG-636302 and ENSBTAG00000010444 and 785655
-
