Tetraspanin Exo-Flow Combo capture kit (Magnetic streptavidin beads, CD9, CD63, and CD81-biotin capture antibodies, Wash and Elution Buffers, Exo-FITC stain)

  • Catalog number
    EXOFLOW150A-1
  • Price
    Please ask
  • Size
    10 reactions
  • Shipping Temperature
    Blue Ice
  • Product category
    Exosomes
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. This Systembio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Systembio FITC is highly sensitive to pH extremes.
  • Conjugation
    Biotinylated, Anti-FITC Antibody, Magnetic beads
  • Test
    Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
  • Gene target
    Tetraspanin   Exo   Combo   capture   kit   Magnetic   streptavidin   CD9   CD63   CD81   capture   antibodies   Elution   Buffers   Exo   stain  
  • Gene symbol
    CD81-AS1, CD63-AS1, CD63, CD81, CD9
  • Short name
    Tetraspanin Exo- Combo capture kit (Magnetic streptavidin , CD9, CD63, CD81- capture antibodies, Elution Buffers, Exo-FITC stain)
  • Technique
    FITC, Flow, All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet. Fluorescein
  • Label
    Magnetic
  • Alternative name
    Tetraspanin Exo-Flow Combo capture reagent (Magnetic biotinilated-binding streptavidin particles, CD9 molecule, CD63 molecule, and CD81 molecule-biotinilated capture antibodies, washing and Elution Buffers, Exo-fluorecein staining)
  • Alternative technique
    fluorescine, kits
  • Alternative to gene target
    CD9 molecule, BTCC-1 and DRAP-27 and MIC3 and MRP-1 and TSPAN-29 and TSPAN29, CD9 and IDBG-13612 and ENSG00000010278 and 928, protein binding, Extracellular, Cd9 and IDBG-189938 and ENSMUSG00000030342 and 12527, CD9 and IDBG-640522 and ENSBTAG00000014764 and 280746
Gene info
Gene info
  • Identity
    HGNC:55455
  • Gene
    CD63-AS1
  • Long gene name
    CD63 antisense RNA 1
  • Locus
    12q13.2
  • Discovery year
    2021-01-20
  • Entrez gene record
    105369779
  • Classification
    • Antisense RNAs
Gene info
Gene info
Gene info
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Tetraspanin Exo Combo capture kit Magnetic streptavidin CD9
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