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Type
Conjugated Primary Antibody
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Conjugated with
Cy7
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
EYA1
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Specificity
This antibody reacts specifically with EYA1 Cy7
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Modification
No modification has been applied to this antibody
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Modification site
None
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
Cytoplasm, Nucleus
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Antigen Source
KLH conjugated synthetic peptide derived from human EYA1
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Gene ID
2138
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Swiss Prot
Q99502
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Human (Homo sapiens), Rat (Rattus norvegicus)
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Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information
Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Has also phosphatase activity with proteins phosphorylated on Ser and Thr residues (in vitro). Required for normal embryonic development of the craniofacial and trunk skeleton, kidneys and ears. Together with SIX1, it plays an important role in hypaxial muscle development; in this it is functionally redundant with EYA2.
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
743nm/767nm
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Synonyms
BOP; BOR; BOS1; OFC1; Eyes absent homolog 1; EYA1
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Also known as
EYA1 Polyclonal Antibody Cy7
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Other name
Anti-EYA1 Polyclonal Cy7
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Conjugated
These antibodies are excite for emission at 650 nm and detected at a 676 nm wavelengths.
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Conjugation
Cy7
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbol
EYA1
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Short name
Anti-EYA1 Cy7
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Technique
Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Immunoglobulin G (IgG)
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Label
Cy7
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Alternative name
EYA1 Polyclonal Antibody, Cy7 Conjugated
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
eyes absent homolog 1 (Drosophila), BOP and BOR and BOS1 and OFC1, EYA1 and IDBG-25014 and ENSG00000104313 and 2138, metal ion binding, nuclei, Eya1 and IDBG-134456 and ENSMUSG00000025932 and 14048, EYA1 and IDBG-644711 and ENSBTAG00000011298 and 511188
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