DLD, human recombinant

• Catalog number
  7829-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Dihydrolipoyl dehydrogenase, mitochondrial, DLDH, E3, GCSL, LAD, PHE3
• Alternative_names
  Dihydrolipoyl dehydrogenase, mitochondrial, DLDH, E3, GCSL, LAD, PHE3
• Description
  A flavin-dependent oxidoreductase
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity: > 50 units/ml. One unit will reduce 1.0 µmole of DL-dihydrolipoamide per minute at pH 7.4 at 25ºC.
• Results
  Specific activity: > 50 units/ml
• Unit Definition
  One unit will reduce 1.0 µmole of DL-dihydrolipoamide per minute at pH 7.4 at 25ºC.
• Molecular Weight
  54.4 kDa (511 aa, 36-509 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1 mM DTT and 0.1 M NaCl
• Background Information
  DLD (Dihydrolipoamide dehydrogenase), also known as GCSL (glycine cleavage system L protein), is a component of the glycine cleavage system as well as of the alpha ketoacid dehydrogenase complexes. DLD is a flavin-dependent oxidoreductase and functions as a component of the alpha-keto acid dehydrogenase, the pyruvate dehydrogenase, the alpha-ketoglutarate dehydrogenase, the branched-chain alpha-keto acid dehydrogenase and as the L protein in the mitochondrial glycine cleavage system. Mutations in DLD protein can result in MSuD (maple syrup urine disease) and congenital infantile lactic acidosis. Recombinant human DLD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMADQ PIDADVTVIG SGPGGYVAAI KAAQLGFKTV CIEKNETLGG TCLNVGCIPS KALLNNSHYY HMAHGKDFAS RGIEMSEVRL NLDKMMEQKS TAVKALTGGI AHLFKQNKVV HVNGYGKITG KNQVTATKAD GGTQVIDTKN ILIATGSEVT PFPGITIDED TIVSSTGALS LKKVPEKMVV IGAGVIGVEL GSVWQRLGAD VTAVEFLGHV GGVGIDMEIS KNFQRILQKQ GFKFKLNTKV TGATKKSDGK IDVSIEAASG GKAEVITCDV LLVCIGRRPF TKNLGLEELG IELDPRGRIP VNTRFQTKIP NIYAIGDVVA GPMLAHKAED EGIICVEGMA GGAVHIDYNC VPSVIYTHPE VAWVGKSEEQ LKEEGIEYKV GKFPFAANSR AKTNADTDGM VKILGQKSTD RVLGAHILGP GAGEMVNEAA LALEYGASCE DIARVCHAHP TLSEAFREAN LAASFGKSIN F
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  DLD  
• Gene symbol
  DLD
• Short name
  DLD, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  DLD, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:2898
• Gene
  DLD
• Long gene name
  dihydrolipoamide dehydrogenase
• Synonyms gene
  • LAD
  • GCSL
• Synonyms gene name
  • dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex)
• Synonyms
  • DLDH
  • E3
  • OGDC-E3
• Synonyms name
  • E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex
• GenBank acession
  • AB209703
• Locus
  7q31.1
• Discovery year
  1988-05-11
• Entrez gene record
  1738
• RefSeq identity
  • NM_000108
• Classification
  • Pyruvate dehydrogenase complex
• VEGA ID
  OTTHUMG00000154813

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products