Protein L
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Catalog number6530-1
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PricePlease ask
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Size1 mg
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SynonymsProtein L
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Alternative_namesProtein L
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DescriptionGenetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Binding CapacityUnder optimal conditions, 1 mg protein L will bind approximately 5 mg human IgG. Optimal binding of Protein L to antibodies occurs at pH 7.5 over a pH range of 2.5 to 3.0.
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Molecular Weight43.2 kDa with 5 IgG binding sites
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized with no additives
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Reconstitution InstructionsReconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.
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Background InformationProtein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody’s antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L can be used to detect, quantify and purify antibodies and antibody/antigen complexes. BioVision’s Recombinant Protein L contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein L detection using anti-His-tag antibodies. Protein L binds to all classes of Ig (IgG, IgM, IgA, IgE, and IgD). Protein L will also bind Single Chain Variable Fragments (ScFv) and Fab Fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Gene target
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Gene symbolLCP1, PGLYRP2, ATRN
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Short nameProtein L
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Alternative nameProtein L
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Gene info
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Identity
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Gene
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Long gene namelymphocyte cytosolic protein 1
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Synonyms gene name
- lymphocyte cytosolic protein 1 (L-plastin)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- EF-hand domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepeptidoglycan recognition protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-03-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Peptidoglycan recognition proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameattractin
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-10-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
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VEGA ID