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Synonyms
COMP, TSP5, EDM1, EPD1, MED, PSACH, THBS5.
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Alternative_names
COMP, TSP5, EDM1, EPD1, MED, PSACH, THBS5.
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Description
A marker of cartilage turnover
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells, when rhCOMP / Thrombospondin‑5 immobilized at 1 µg/well. More than 45% of ATDC-5 cell adhesion will be induced.
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Results
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells, when rhCOMP / Thrombospondin‑5 immobilized at 1 µg/well. More than 45% of ATDC-5 cell adhesion will be induced.
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Molecular Weight
This protein fused with 6×His tag at the C-terminus, has a calculated MW of 82 kDa. The predicted N-terminus is Gln 21. DTT-reduced Protein migrates as 110-130 kDa due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
Cartilage oligomeric matrix protein (COMP) also known as Thrombospondin-5 (TSP5), EDM1, EPD1, MED, PSACH, THBS5, which belongs to the thrombospondin family. COMP / TSP5 contain 4 EGF-like domains, 1 TSP C-terminal (TSPC) domain, 8 TSP type-3 repeats. Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. COMP may play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. COMP can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Thrombospondin-5 could play a role in the pathogenesis of osteoarthritis. COMP is a marker of cartilage turnover.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
COMP
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Short name
CellExp™ COMP/TSP5, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ cartilage oli this GO meric matrix protein/TSP5, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
cartilage oli this GO meric matrix protein, EDM1 and EPD1 and MED and PSACH and THBS5, COMP and IDBG-38816 and ENSG00000105664 and 1311, heparan sulfate proteoglycan binding, Extracellular, Comp and IDBG-162847 and ENSMUSG00000031849 and 12845, BT.3034 and IDBG-641613 and ENSBTAG00000004630 and 281088
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