Tubulin, beta, Isotype III, Clone: TU-20, Mouse Monoclonal antibody-Human, Syrian Hamster, Rat, Mouse, Baboon, Bovine, Swine, FITC; flow

  • Catalog number
    YSRTMCA2047F
  • Price
    Please ask
  • Size
    0.1 mg
  • Alternative name 1
    Tubulin, beta, Isotype III, Clone: TU-20, Mab anti-Human, Syrian Hamster, Rat, Mouse, Baboon, Bovine, Swine, FITC; flow
  • Alternative name 2
    Tubulin, beta, Isotype III, Clone: TU-20, Mab antibody-Human, Syrian Hamster, Rat, Mouse, Baboon, Bovine, Swine, FITC; flow
  • Alternative name 3
    Tubulin, beta, Isotype III, Clone: TU-20, Mab antibody-Homo sapiens, Syrian Hamster, Rattus norvegicus, Mus musculus, Baboon, Bovine, Swine, FITC; flow
  • Alternative name 4
    anti-Tubulin beta Isoty III Clone: TU-20 Syrian flow
  • Alternative name 5
    Tubulin beta Isoty III Clone: TU-20 Syrian flow
  • Clonality
    Monoclonal Antibody
  • Description
    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
  • Storage and handling
    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
  • Advisory
    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
  • Properties
    If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Most human antibodies and protein are very homologous to baboon genes. BLASTed sequences are used to find homological regions. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Conjugation
    Anti-FITC Antibody
  • Test
    Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Group
    isotypes
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Mus musculus, Rattus norvegicus
  • French translation
    anticorps
  • Gene target
    Tubulin   beta   III   TU-20   Monoclonal   Syrian   Swine  
  • Gene symbol
    PIRC103, SNORD114-20, SNORD116-20, IGLVI-20, PIRC104, IGHV3-20, CPLX3, SNORD115-20
  • Short name
    Tubulin, beta, Isotype III, Clone: TU-20, Mouse Monoclonal antibody- , Syrian , , Mouse, , , Swine, FITC;
  • Technique
    Mouse monoclonal, Antibody, Isotype, Mouse, FITC, Flow, antibodies against human proteins, antibodies for, Fluorescein, Isotypes and controls, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Label
    FITC
  • Species
    Bovine, Baboons, Bos Taurus genes are available form the Bovine Genome database. Hamsters, Humans, Mouses, Rats
  • Alternative name
    Tubulin, b, isoform III, clonality: TU-20, Mouse monoclonal (antibody to-)-H. sapiens, Syrian Hamster, Rat, Mouse, Baboon, Bovine, swine, fluorecein; flow
  • Alternative technique
    antibodies, control, murine, fluorescine
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 103
  • Locus
    20
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin lambda variable (I)-20 (pseudogene)
  • Synonyms gene name
    • immunoglobulin lambda variable (I)-20
    • immunoglobulin lambda variable (I)-20 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-05-08
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin lambda locus at 22q11.2
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 104
  • Locus
    20
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
  • Tree numbers
    • E01.370.225.500.363.342
    • E01.370.225.500.386.350
    • E05.196.712.516.600.240.350
    • E05.200.500.363.342
    • E05.200.500.386.350
    • E05.242.363.342
    • E05.242.386.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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