GRK2, 3, CT (G Protein Coupled Receptor Kinase 2, Beta-adrenergic Receptor Kinase 1, ADRBK1, Beta-ARK1, Beta-ARK-1, BARK1, BARK/G Protein Coupled Receptor Kinase 3, Beta-adrenergic Receptor Kinase 2, ADRBK2, Beta-ARK-2, BARK2) (FITC) Antibody
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Catalog number
GEN643531
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Price
Please ask
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Size
100 Tests
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Also known as
GRK2, 3
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Other names
N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2ak
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Clone
13A189
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes human GRK2,3. Species sequence homology: rat.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein G affinity chromatography.
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Form Appearance
Supplied as a liquid in PBS, 0.1% sodium azide, 15mg/ml BSA. Labeled with fluorescein isothiocyanate (FITC).
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer. FITC conjugates are sensitive to light.
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Tested applications
Flow Cytometry (FC/FACS)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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French translation
anticorps
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Gene target
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Gene symbol
GRK2, GRK3, AURKA, AXL, RNF111, IGHVIII-2-1, MIR1-2, AURKB, TRUND-NNN9-1, SLC2A4RG
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Short name
GRK2, 3, CT (G Protein Coupled Receptor Kinase 2, Beta-adrenergic Receptor Kinase 1, ADRBK1, Beta-ARK1, Beta-ARK-1, BARK1, BARK/G Protein Coupled Receptor Kinase 3, Beta-adrenergic Receptor Kinase 2, ADRBK2, Beta-ARK-2, BARK2) (FITC) Antibody
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
Anti-GRK2, 3, CT (G Protein Coupled Receptor Kinase 2, Beta-adrenergic Receptor Kinase 1, ADRBK1, Beta-ARK1, Beta-ARK-1, BARK1, BARK/G Protein Coupled Receptor Kinase 3, Beta-adrenergic Receptor Kinase 2, ADRBK2, Beta-ARK-2, BARK2) (FITC)
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Alternative technique
antibodies, fluorescine
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Alternative to gene target
adrenergic, beta, receptor kinase 1, BARK1 and BETA-ARK1 and GRK2, ADRBK1 and IDBG-59896 and ENSG00000173020 and 156, transferase activity, Plasma membranes, Adrbk1 and IDBG-129419 and ENSMUSG00000024858 and 110355, ADRBK1 and IDBG-644669 and ENSBTAG00000005832 and 282682
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Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
aurora kinase A
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Synonyms gene
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Synonyms gene name
- serine/threonine kinase 15
- serine/threonine kinase 6
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-10-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
ring finger protein 111
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Synonyms
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GenBank acession
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Locus
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Discovery year
2003-05-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
microRNA 1-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
aurora kinase B
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Synonyms gene
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Synonyms gene name
- serine/threonine kinase 12
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1999-01-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chromosomal passenger complex
- Protein phosphatase 1 regulatory subunits
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
SLC2A4 regulator
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2001-06-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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