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Anti-A Raf (Ser214) (Polyclonal), ALEXA Fluor 594

Anti-A Raf (Ser214) (Polyclonal), ALEXA Fluor 594 is available 1 time from Bioss polyclonal antibodies labs

GENTObs-3005R-A594 | Anti-A Raf (Ser214) (Polyclonal), ALEXA Fluor 594 size: 100 microliters | 615.90 USD

Catalog number GENTObs-3005R-A594
Price 615.90 USD
Size 100 microliters
Type Conjugated Primary Antibody
Conjugated with ALEXA FLUOR® 594
Host organism Rabbit (Oryctolagus cuniculus)
Target Protein/Peptide A Raf Ser214
Specificity This antibody reacts specifically with A Raf (Ser214)
Modification Phosphorylation
Modification site Ser214
Clonality Polyclonal Antibody
Clone Polyclonal Antibodies
Concentration 1ug per 1ul
Subcellular locations N/A
Antigen Source KLH conjugated synthetic phosphopeptide derived from human A Raf around the phosphorylation site of Ser214
Gene ID 369
Swiss Prot N/A
Applications IF(IHC-P)
Applications with corresponding dilutions IF(IHC-P)(1:50-200)
Cross reactive species Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species details No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Background information The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
Purification method Purified by Protein A.
Storage Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Excitation emission 590nm/617nm
Synonyms A raf 1; A Raf proto oncogene serine/threonine protein kinase; ARAF 1; ARAF; ARaf proto oncogene serine/threonine protein kinase; ARAF1; Oncogene Araf1; Oncogene PKS2; PKS 2; PKS; PKS2; Proto oncogene Pks; RAFA 1; RAFA1; Ras binding protein DA Raf; v raf murine sarcoma 3611 viral oncogene homolog; v raf murine sarcoma 3611 viral oncogene homolog 1; v raf oncogene homolog 1 murine sarcoma 3611 virus; ARAF_HUMAN.
Also known as A Raf (Ser214) Polyclonal Antibody
Other name Anti-A Raf (Ser214) Polyclonal
Advisory Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Properties For facs or microscopy Alexa 1 conjugate.
Conjugation Alexa Fluor,ALEXA FLUOR® 594
Conjugated Alexa conjugate 1
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Gene targetRaf Ser214
Short name Anti-A Raf (Ser214)
Technique Polyclonal, anti
Isotype Immunoglobulin G (IgG)
Label ALEXA FLUOR 594
Alternative name A Raf (Ser214) Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
Alternative technique polyclonals
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Anti-A Raf (Ser214) | Technique alternative | 01010268620
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