HDAC3 Antibody

• Catalog number
  R30679
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  HDAC3
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, IHC-F, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the HDAC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This HDAC3 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O15379
• Purity
  Antigen affinity
• Description
  Histone deacetylase 3 is a member of the histone deacetylase/acuc/apha family of proteins and is an enzyme that in humans is encoded by the HDAC3 gene. The enzyme has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. The protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. And this gene is regarded as a potential tumor suppressor gene. HDAC3 has an open reading frame of 428 amino acids and shares 53% amino acid identity with HDAC1 and 52% with HDAC2. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. All experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 resulted in loss of enzymatic activity associated with HDAC4.
• Immunogen
  Amino acids 411-428 (NEFYDGDHDNDKESDVEI) were used as the immunogen for this HDAC3 antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the HDAC3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  HDAC3  
• Gene symbol
  HDAC3
• Short name
  Anti-HDAC3
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to HDAC3
• Alternative technique
  antibodies
• Alternative to gene target
  histone deacetylase 3, HD3 and RPD3 and RPD3-2, HDAC3 and IDBG-50725 and ENSG00000171720 and 8841, NAD-dependent histone deacetylase activity (H3-K18 specific), nuclei, Hdac3 and IDBG-140814 and ENSMUSG00000024454 and 15183, HDAC3 and IDBG-646121 and ENSBTAG00000017360 and 404125

Gene info

• Identity
  HGNC:4854
• Gene
  HDAC3
• Long gene name
  histone deacetylase 3
• Synonyms
  • RPD3
  • HD3
  • RPD3-2
  • KDAC3
• GenBank acession
  • AF059650
• Locus
  5q31.3
• Discovery year
  1999-01-29
• Entrez gene record
  8841
• Pubmed identfication
  • 9501169
  • 9464271
• RefSeq identity
  • NM_003883
• Classification
  • NCoR/SMRT transcriptional repression complex subunits
  • Histone deacetylases, class I
• VEGA ID
  OTTHUMG00000129629

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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