-
Synonyms
CD141, BDCA-3, THBD, TM
-
Alternative_names
CD141, BDCA-3, THBD, TM
-
Description
An endothelial cell expressed transmembrane glycoprotein
-
Recombinant
Yes
-
Source
HEK 293 cells
-
Purity by SDS PAGE
≥98%
-
Assay
SDS-PAGE
-
Endotoxin Level
< 0.1 ng/ug of protein (
-
Activity Specifications test method
Measured by its ability to activate protein C induced cleavage of the chromogenic substrate, BOC-Asp-Pro Arg-AMC in the presence of thrombin. The specific activity is >500 pmoles/min/µg.
-
Biological activity
Measured by its ability to activate protein C induced cleavage of the chromogenic substrate, BOC-Asp-Pro Arg-AMC in the presence of thrombin. The specific activity is >500 pmoles/min/µg.
-
Molecular Weight
51.4 kDa
-
Storage Temp
-20°C
-
Shipping
Gel Pack
-
Shelf Life
12 months
-
Appearance
Lyophilized powder
-
Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium phosphate, pH 7.5.
-
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
-
Background Information
Thrombomodulin (TM, CD141, and THBD) is an endothelial cell expressed transmembrane glycoprotein that can form a complex with the coagulation factor, Thrombin. The Thrombomodulin/Thrombin complex converts protein C to its activated form, protein Ca, which in turn proteolytically cleaves and deactivates factor Va and factor VIIIa, two essential components of the coagulation mechanism. This inactivation reduces the generation of additional thrombin and thereby effectively prevents continued coagulation. Reduced levels of Thrombomodulin can correlate with the pathogenesis of certain cardiovascular diseases, such as atherosclerosis and thrombosis. However, the serum levels of the truncated circulating form of Thrombomodulin are typically elevated during inflammation and in the presence of various inflammatory related diseases. Recombinant soluble Thrombomodulin is a 491 amino acid glycoprotein containing the extracellular domain of Thrombomodulin.
-
Amino acid sequence
APAEPQPGGS QCVEHDCFAL YPGPATFLNA SQICDGLRGH LMTVRSSVAA DVISLLLNGD GGVGRRRLWI GLQLPPGCGD PKRLGPLRGF QWVTGDNNTS YSRWARLDLN GAPLCGPLCV AVSAAEATVP SEPIWEEQQC EVKADGFLCE FHFPATCRPL AVEPGAAAAA VSITYGTPFA ARGADFQALP VGSSAAVAPL GLQLMCTAPP GAVQGHWARE APGAWDCSVE NGGCEHACNA IPGAPRCQCP AGAALQADGR SCTASATQSC NDLCEHFCVP NPDQPGSYSC MCETGYRLAA DQHRCEDVDD CILEPSPCPQ RCVNTQGGFE CHCYPNYDLV DGECVEPVDP CFRANCEYQC QPLNQTSYLC VCAEGFAPIP HEPHRCQMFC NQTACPADCD PNTQASCECP EGYILDDGFI CTDIDECENG GFCSGVCHNL PGTFECICGP DSALARHIGT DCDSGKVDGG DSGSGEPPPS PTPGSTLTPP A
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants