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Long name
Rabbit Antibody against Human Acetyl-HIST1H2BB(K5), Non-conjugated
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Synonym name
Acetyl-HIST1H2BB(K5) Human Antibody
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Species reactivity
Human
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Tested applications recommended dilutions
ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200
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Conjugation
Non-conjugated
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Antibody isotype
IgG
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UniProt ID
P33778
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
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Purity
>95%
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Purification method
Antigen Affinity Purified Antibodies
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Antibody category
Polyclonal Antibodies
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Immunogen type
Peptide sequence around site of Acetyl-Lys (5) derived from human Histone H2B type 1-B.
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Immunogen species
Human
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Alias
Histone H2B type 1-B, Histone H2B.1, Histone H2B.f, H2B/f, HIST1H2BB, H2BFF
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Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Clonality
Polyclonal antibody
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Shipping and storage
Acetyl-HIST1H2BB(K5) Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigen
Histone H2B type 1-B
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Description
N terminal acetylation or CH3CO as epigenetic regulation of Anti-Acetyl-HIST1H2BB(K5) by NATs. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps