Amplite Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*
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Catalog number
13621
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Price
Please ask
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Size
200 Tests
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Description
Amplite Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence* is a kit that provides the most sensitive method for detecting neutral SMase activity or screening its inhibitors. This kit uses Amplite Red as a fluorogenic probe to indirectly quantify the phosphocholine produced from the hydrolysis of sphingomyelin (SM) by sphingomyelinase (SMase). It can be used for measuring the SMase activity in blood, cell extracts or other solutions.
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Shipping
Dry Ice
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Storage
Freezer
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Shelflife
6 months
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Chemical Components
Component A: Enzyme Mix ; Component B: Sphingomyelin ; Component C: Amplite™ Red ; Moderate Component D: SMase Reaction Buffer ; Component E: Assay Buffer ; Component F: Sphingomyelinase Standard ; High Component G: DMSO
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Excitation Wavelength
571 nm
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Emission Wavelength
585 nm
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Personal precautions
Use appropriate protective equipment and methods to clean up spilled substances promptly.
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Uses
Laboratory chemicals *For Research Use Only*
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Ordering
To order Amplite Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*, please use the Cat. Nr.13621 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Amplite Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*
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Test
aat supplies other types of Assays as 1. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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Conjugation
red dye
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Gene target
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Short name
Amplite Fluorimetric Sphingomyelinase Assay Kit *Red *
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Technique
Assay, assays
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Host
Assay
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Label
Red
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Alternative name
Amplite fluorimetry Sphingomyelinase test reagent *Red Fluorescence*
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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