Human Recombinant Alpha-Enolase
-
Catalog number
6363-500
-
Price
Please ask
-
Size
500 ug
-
-
Synonyms
Human Recombinant Alpha-Enolase
-
Alternative_names
Non-Neuronal Enolase (NNE); Enolase-1(ENO1); MBP-1; MYC promoter-binding protein 1; PPH; taucrystallin.
-
Description
A metalloenzyme responsible for conversion of 2-PG to PEP
-
Recombinant
Yes
-
Source
E. Coli
-
Purity by SDS PAGE
≥90%
-
Assay
SDS-PAGE
-
Biological activity
≥ 80 U/mg
-
Results
≥ 80 U/mg
-
Unit Definition
One unit is the amount of enzyme that will generate 1.0 nmole of H2O2 per minute at pH 7.2 and 25 ̊C.
-
Molecular Weight
47.1 kDa (1-434 aa)
-
Storage Temp
-20°C
-
Shipping
gel pack
-
Shelf Life
12 months
-
Concentration
1 mg/ml
-
Appearance
Liquid
-
Physical form description
In 50 mM Tris, 150 mM NaCl, 20 mM MgCl2 and 20% Glycerol
-
Background Information
Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an auto antigen associated with Hashimoto's encephalopathy and severe asthma.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Additional description
The Recombinant Alpha-Enolase is a α- or alpha protein sometimes glycoprotein present in blood.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
ENO1, ENO1P1, ENO1P2
-
Short name
Recombinant Alpha-Enolase
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Species
Human, Humans
-
Alternative name
H. sapiens Rec. a-Enolase
-
Alternative technique
rec
-
Gene info
-
Identity
-
Gene
-
Long gene name
enolase 1
-
Synonyms gene
-
Synonyms gene name
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1989-04-21
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Enolases
- MicroRNA protein coding host genes
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene name
enolase 1 pseudogene 1
-
Synonyms gene
-
Synonyms gene name
- enolase 1, (alpha) pseudogene
- enolase 1, (alpha) pseudogene 1
-
GenBank acession
-
Locus
-
Discovery year
1992-11-04
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
enolase 1 pseudogene 2
-
Synonyms gene name
- enolase 1, (alpha) pseudogene 2
-
Locus
-
Discovery year
2010-03-11
-
Entrez gene record
-
RefSeq identity
-
VEGA ID
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
