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Anti-KLH (Polyclonal), ALEXA Fluor 594

Anti-KLH (Polyclonal), ALEXA Fluor 594 is available 1 time from Bioss polyclonal antibodies labs

GENTObs-0225M-A594 | Anti-KLH (Polyclonal), ALEXA Fluor 594 size: 100 microliters | 579.76 USD

Catalog number GENTObs-0225M-A594
Price 579.76 USD
Size 100 microliters
Type Conjugated Primary Antibody
Conjugated with ALEXA FLUOR® 594
Host organism Mouse (Mus musculus)
Target Protein/Peptide KLH
Specificity This antibody reacts specifically with KLH
Modification No modification has been applied to this antibody
Modification site None
Clonality Polyclonal Antibody
Clone Polyclonal Antibodies
Concentration 1ug per 1ul
Subcellular locations N/A
Antigen Source KLH protein
Gene ID N/A
Swiss Prot N/A
Applications IF(IHC-P)
Applications with corresponding dilutions IF(IHC-P)(1:50-200)
Cross reactive species Others
Cross Reactive Species details Keyhole limpet hemocyanin (KLH)
Background information Keyhole limpet hemocyanin is an extremely large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000 in aggregates with molecular weights of 4,500,000-13,000,000. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata.Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of for research, biotechnology and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production.[2] KLH is the most widely employed carrier proteins for this purpose. KLH is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens, allows a high hapten:carrier protein ratio increasing the likelihood of generating hapten-specific . In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically based research techniques in mammalian model organisms.KLH may also be a challenging molecule to work with because of its propensity to aggregate and precipitate. Aggregates remain immunogenic, but limit the ability to conjugate haptens and are difficult to manipulate in the laboratory.
Purification method Purified by Protein A.
Storage Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Excitation emission 590nm/617nm
Synonyms Keyhole limpet hemocyanin KLH
Also known as KLH Polyclonal Antibody
Other name Anti-KLH Polyclonal
Advisory Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Properties For facs or microscopy Alexa 1 conjugate.
Conjugation Alexa Fluor,ALEXA FLUOR® 594
Conjugated Alexa conjugate 1
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Gene targetKLH
Short name Anti-KLH
Technique Polyclonal, anti
Isotype Immunoglobulin G (IgG)
Label ALEXA FLUOR 594
Alternative name KLH Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
Alternative technique polyclonals
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